Liver zymogram and functional indexes in workers exposed to arsenic 职业接触砷工人肝功酶谱及肝功能变化
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Effect of aluminum chelating agent on liver zymogram and on essential elements of rats induced by aluminum 铝螯合剂对染铝大鼠肝酶谱及必需元素影响
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According to current studies , there are four hypothesis about mechanism : damage to vascular system , zymogram alteration , androgenic hormone disturbance and lipid peroxidation 目前研究认为存在四种关于作用机理的假设：血管系统损伤、酶谱改变、雄性激素失调和脂质过氧化。
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By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ) , zymogram of 23 species of 11 genera in 4 subfamilies mirinae , orthotylinae , phylinae and deraeocorinae are gained . by specific primer pcr and sequencing , 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae , orthotylinae , phylinae , deraeocorinae , bryocorinae from 64 species of 23 genera which involved in this research , and 2 outgroup species of family anthocoridae as well . 1 首次通过特异引物扩增和基因测序的研究方法从被试的5亚科23个属的64种盲蝽中获得了盲蝽亚科、合垫盲蝽亚科、叶盲蝽亚科、赤爪盲蝽亚科和蕨盲蝽亚科bryocorinae5个亚科17个属的34种盲蝽以及外群花蝽科anthocoridae2种花蝽的cytb基因序列57条。
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The peroxidase activity of variant t220x was obviously higher than acceptor lu22 at seedling stage . the result of peroxidase electrophoresis indicated that there were difference both in the depth and in the amount of zymogram between lu22 and t220x . . so it was concluded that the variety of peroxidase was the result of change of heriditary substance 小麦幼苗期过氧化物酶活性的测定显示，变异体t220x的酶活性高于受体鲁22 ；过氧化物酶电泳显示， t220x和鲁22过氧化物酶在酶带深浅和数目方面都存在差异，说明外源遗传物质已影响到过氧化物酶的变化。
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The results revealed that : ( 1 ) the zymogram of est isozyme in the livers from all microtus fortis existed widespread polymorphism . they were similar to each other at the i area while quite different at the iii and iv area . at the ii area , the voles from hunan , ningxia and the big voles from heilongjiang were close but the difference between the small voles from heilongjiang and three other groups was obviously 结果表明： ( 1 )东方田鼠的肝est同工酶活性较强，有着丰富的多态性，四类鼠在区较相近，在区和区差异都较大，在区湖南、宁夏和黑龙江大体型东方田鼠较为相似而黑龙江小体型东方田鼠与它们差别较大。
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The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands , which exactly demonstrated at protein level that a . niger j 506 was surely a mutant of a . niger m1 . zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture , while two xylanases in initial strain . what is important , the third xylanase in a . niger j 506 have higher activity and more production levels from page and zymogram of xylanase 尤其是在木聚糖酶谱带检测中发现，突变株发酵液中有三种类型的木聚糖酶，而出发菌株中只有两种类型的木聚糖酶，并且通过考马斯亮蓝g250染色和琼脂糖板上的透明圈发现，突变株中第三种类型的木聚糖酶不仅表达量很大，活力也很高。
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2 . the data from zymogram ( est bands and their rf ) are transferred into bimorphic characteristic data . a data matrix is established based on the data and calculated by phylogenetic analysis software package winclada 1 . 00 . 04 , and then phylogenetic trees of intraspecies and interspecies are mapped 2 、将所获得的盲蝽科4亚科11属23种盲蝽雄雌个体的酶谱数据(酶带和相对迁移率rf )转化为二态特征数据建立矩阵，应用系统发育分析软件包winclada1 . 00 . 04对数据矩阵进行分析运算，并分别获得各个亚科内和亚科间的系统发育树。
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The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea , which characterized by low temperature , high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate , self - supporting stocks of krill . 2 genetic diversity and differentiation of p . latifrons specimens of p . latifrons were collected from the east china sea and the south china sea . the zymogram phenotypes of aspartate aminotransferase ( e . c . 2 . 6 . 1 . 1 , aat ) , alkaline phosphatase ( e . c . 3 . 1 . 3 . 1 , alp ) , a - amylase ( a - amy ) , r - amylase ( r - amy ) , esterase ( est ) , lactate dehydrogenase ( ldh ) , raalate dehydrogenase ( mdh ) , malic enzyme ( me ) , and phosphoglweoisomerase ( pgi ) were scored (二)宽额假磷虾遗传多样性和遗传分化研究1 .本文对东海外海和南海2个站位宽额假磷虾群体进行了分析，在检测的9个酶系统中，共检测到11个酶位点:天冬氨酸转氨酶( l个位点， 2个等位基因) ，碱性磷酸酶( 2个位点， a加了和a加2各有2个等位基因) ， r淀粉酶( l个位点， 2个等位基因) ，醋酶( 2个位点， es巧和est7各有2个等位基因) ，苹果酸脱氢酶( l个位点， 3个等位基因) ，苹果酸酶( l个位点， 2个等位基因) ，乳酸脱氢酶( l个位点， 4个等位基因) ，磷酸葡萄糖转氨酶( l个位点， 3个等位基因) ; a淀粉酶为单态。
