| 1. | Study of culture conditions for streptomyces
链霉亲和素产生菌培养条件的研究 |
| 2. | Study on the spectral change of cate quantum dots synthesized in aqueous solution labeling avidin by uv and fluorescence spectrophotometries
量子点标记亲和素前后光谱变化的研究 |
| 3. | Surface plasmon resonance technique ; immunosensor ; amplified assay ; streptavidinbiotinylated antibody complex
表面等离子体共振技术免疫传感器放大检测链霉亲和素-生物素化抗体复合物 |
| 4. | It increases the detectable rate of antigen and sensitivity by applying biotin - avidin system to p24 antigen detection system
将生物素-亲和素系统应用到p24抗原检测系统，提高了抗原检出率和假阳性。 |
| 5. | ( 2 ) an amplified immunoassay with improved sensitivity has been proposed based on two - step amplification procedures of enzyme - catalyzed precipitation and biotin - streptavidin binding system ( in chapter 3 )
( 2 )提出了一种基于酶催化沉积底物和生物素-亲和素两步放大的超灵敏免疫检测方法(第3章) 。 |
| 6. | Biotin - avitin system ( bas ) was used to immobilize dna on the pdms channel compared to the direct immobilization methds . the hybridization reaction also has been examined between immobilized probe and target dna
针对pdms新型材料，试验了两种dna分子的固定方法，确定了生物素?亲和素介导的dna分子固定化方法，并使用此芯片和固定方法进行了芯片上的dna杂交反应。 |
| 7. | We have demonstrated isolation of single - class plasma lipoproteins based on the immunological method associated with streptavidin ? biotin capture technology , and the lipoprotein cholesterol was consecutively quantified on the same strip without handling of reagents ( 11 )
我们还论证了基于免疫学方法结合链亲和素捕获技术分离单一级别血浆脂蛋白，并且在同一条带上不用对反应物进行操作就能连续定量检测脂蛋白胆固醇。 |
| 8. | Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a . biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
抗体通过单层蛋白a固定到金电极表面,预先处理好的生物素化抗体和辣根过氧化物酶( hrp )标记的亲和素形成的网状复合物作为免疫反应信号的放大探针。 |
| 9. | Surface plasmon resonance immunosensor is a relatively new immunoassay technique and has been receiving more and more attention in recent years . however , a major disadvantage of spr for bioanalytical applications is that low concentration or low molecular mass analytes could not be detected directly . therefore , it is a challenging task to develop strategies for improving the detection limit sensitivity of spr . in this paper , authors present a novel strategy for improving the sensitivity of spr immunosensing using streptavidinbiotinylated antibody complex . it is proven that the amplification strategy causes a dramatic improvement of the detection sensitivity . this amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein . the complex can be formed in a crosslinking network of molecules so that the amplification of response signal will be realized due to the big molecular size of complex
将链霉亲和素-生物素系统用于表面等离子体共振免疫传感的信号放大，实时检测了人免疫球蛋白g higg的蛋白浓度。发生免疫反应的传感片和生物素化抗体反应后，传感片表面的一层生物素分子随后与链霉亲和素-生物素化抗体复合物中的链霉亲和素的活性位点发生亲和反应，从而使传感片表面特异健合的物质质量显著增加，大大提高了免疫检测的灵敏度和检测限。免疫反应经放大后，可检测0 . 00510g ml浓度区间内的higg 。 |
| 10. | There are mang immobilization methods we can use directed or indirected . the use of sam in various fields of research is rapidly growing , a interesting work is based on the strong absorption of disulfides ( r - s - s - r ) , sulfides ( r - s - r ) or thiols ( r - sh ) on the gold surface . while integrating with the new improving in biomolecule immobilization , this thesis presents a theoretical and applied studies of several new piezoelectric immunosensors based on the au - s sams
通常将生物分子固定于石英晶体电极表面或石英晶体表面的惰性载体涂层主要有直接固定法和间接固定法，包括有硅烷化连接法，聚合物膜连接法，非特异性吸附蛋白间接固定法， lb膜技术，生物素-亲和素体系( bas )法以及自组装单分子层( sams )技术等等。 |
