English translation for "体转"

side stretch

Example Sentences:

	1.	Gfp expression was monitored using a fluorescence microscope . the result showed that the fusion gene was expressed at a low level 利用脂质体转染的方法在美国棉铃虫细胞hz中表达了gfp - actin融合基因。
	2.	We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0 我们直接将含有4个内含子和自身信号肽的2 . 4kb全长人生长激素基因直接克隆至真核表达载体pcdna3 . 0 ，然后利用脂质体转染家蚕bmn细胞，瞬时表达hgh 。
	3.	Recombinant fpvs were selected and purified by blue plaques expressing 3 - galactosidase . . the expression of foreign genes in rfpvs were confirmed by ifa . trails for evaluating protective efficacy of rfpvs against very virulent mdv or aiv challenge 纯化后脂质体转染，蓝斑筛选纯化得到稳定的重组病毒rfpv - ps - ha - pe / l - f ，间接免疫荧光法证实， ha基因和f基因同时得到了表达。
	4.	In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv 本研究采用脂质体转染方法，将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞，经6轮蚀斑克隆、筛选、表达， pcr鉴定和dot - elisa检测，证明该重组病毒已构建成功，并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。
	5.	The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue , then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells . after screeened with g418 and cloned , 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level 抽提、鉴定、纯化重组质粒后，脂质体转染法将重组质粒导入中国仓鼠卵巢细胞( cho - dhfr ~ - )中， g418选择转染子并克隆化培养，经rt - pcr和分子灯塔探针杂交鉴定其mrna转录，获得4株稳定表达54位密码突变型mbl的cho细胞。
	6.	After the recombinant plasmid pcdna3 . 1 / ts87 was identified by digestion of hindlll and bamh i , it transformed into cos7 by lipofectamine . expression product was identified by immunohistochemical method , sds - page and western - blot . the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3 . 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas . l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t . s artificially and sera from rabbit immunized with soluble antigen of t . s and with protein expressed by ts87 gene and by a monoclonal antibody of t . s 通过细胞的免疫组化，细胞裂解物的sds - page电泳， westem - blot分析检测目的基因的表达情况。免疫组化结果显示：重组质粒转染的细胞质中有棕褐色颗粒，而空载体转染细胞及正常细胞无此现象；细胞裂解物sds - page电泳结果显示：只有重组质粒转染的细胞在约38kd处有明显的蛋白带，这与理论计算的ts87基因表达蛋白的分子量为38kd基本一致； western - blot分析结果显示：约38kd的蛋白带能够分别被旋毛虫感染兔血清，成虫虫体可溶性抗原免疫兔血清， ts87基因原核表达蛋白免疫兔血清( ts87血清)以及一株具保护性的旋毛虫单抗特异识别。
	7.	2 . construction of chimeric mtb8 . 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8 . 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8 . 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments , respectively 3 .重组质粒在真核细胞中的表达: pm 、 pms 、 pmi和pmsl重组质粒用lipofectaminatmzo0o脂质体转染试剂转染cos一7细胞，进行瞬时表达， 48小时后，用rl ’ - pcr检测目的基因在mrna水平的表达;用westemblotting检测hil一12在蛋白质水平的表达。
	8.	However , only c - myc protein was slightly increased in cells transfected with vector alone and other proteins expression level were the same as conditions in the absence of tgf - 1 . in addition , hepg2 cells transfected with pcdnas . 1 / ha - axudl plasmid were arrested in gl phase compared with the cells transfected with vector alone 另外，流式细胞仪检测表明， ha一axudi蛋白的过表达细胞组较空载体转染细胞组， g0 / g1 ( % )上升，而s ( % )下降，说明细胞阻滞于g1期。
	9.	In order to study the function of cycling2 in vitro culturing cell line , we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent , and studied the function of cycling2 expression on the cell proliferation in vitro , further investigated the regulation mechanism of cycling2 . at the same time , we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue . material and method 1 material : piresneo vector was purchased from clonetech , plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640 , , dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit , mouse monoclonal antibody p21wafl ( in use ) , dab staining kit were purchased from maixin company 实验材料与方法1 .实验试剂高糖dmem 、 rpmll640和胎牛血清购自美国g山eo / brl公司; dmewf12 ( 1 : 1 )混合培养液购自美国hyclone公司;胰蛋白酶购自美国si目叮a公司; hepes由美国amersco公司分装;脂质体转染试剂( upofectalnineplusreageni )和以18为美国玩vitrogen公司产品; piresneo载体购自美国cloneteeh公司;质粒提取及纯化试剂盒购自德国qiagen公司; ultresensitive翎s一p免疫组织化学试剂盒;鼠单克隆抗体户3 ( do一7 )蛋白(即用型) ;鼠单克隆抗体p21waf ， (即用型) ; dab染色试剂盒均购自福建迈新公司;鼠单克隆抗体pziwa曰(浓缩型) ;辣根过氧化酶标记羊抗鼠二抗购自北京中山公司; ecl试剂盒购自美国santacruze公司; dcproteinassay试剂盒购自bi 。

Similar Words：

"体轴系纵轴" English translation, "体轴下的" English translation, "体轴形成" English translation, "体轴原点" English translation, "体珠海猪鱼" English translation, "体壮如牛" English translation, "体锥面" English translation, "体姿" English translation, "体姿稳定" English translation, "体姿协调" English translation