| 1. | Gene cloning provides a means of purifying and propagating specific dna segments .
基因克隆化提供了一种纯化和扩增特定DNA片段的方法。 |
| 2. | Gene cloning provides a means of purifying and propagating specific dna segments
基因克隆化提供了一种纯化和扩增特定dna片段的方法。 |
| 3. | Establishment and characterization of the cloned rabbit mesenchymal stem cell line from bone marrow
克隆化兔骨髓基质干细胞系的建立及其生物学特性 |
| 4. | Cloning and sequence analysis of bos taurus hepatitis c virus core - binding protein 6 homologue gene
牛丙型肝炎病毒核心蛋白结合蛋白6同源基因的克隆化研究 |
| 5. | Cloning and sequencing analysis of sus scrofa hepatitis c virus core - binding protein 6 homologue gene
猪丙型肝炎病毒核心蛋白结合蛋白6同源基因的克隆化研究 |
| 6. | The screening of supernatants was carried out with using indirect elisa . after subcloning by limiting dilution and selection . five clones of cells with high od value were acquired and named mvi
过阳性杂交瘤细胞的筛选和有限稀释法的克隆化步骤，获得了5株od值高的单克隆细胞株，编号为mv1 ， mv2 ， mv3 ， mv4 ， mv5 。 |
| 7. | 2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent
取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞，再常规融合和筛选，三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。 |
| 8. | The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue , then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells . after screeened with g418 and cloned , 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鉴定、纯化重组质粒后，脂质体转染法将重组质粒导入中国仓鼠卵巢细胞( cho - dhfr ~ - )中， g418选择转染子并克隆化培养，经rt - pcr和分子灯塔探针杂交鉴定其mrna转录，获得4株稳定表达54位密码突变型mbl的cho细胞。 |
| 9. | Results : the factors , which might be negatively influencing the progress of military nursing affairs might be sumarized as follows : relatively low level distribution of nursing masses ; tendency of changes in nursing personal composition towardsocialization ; transfering of admistrative resources to the non - specialized positions ; trends of act according to regulations in working mode
结果：军队护理发展负性因素为：群体结构的低层面偏态分布；队伍成分的社会化演绎；管理资源的非专业化流向；工作模式的“克隆化”倾向；管理思维的二值误区。 |
| 10. | Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg , and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti - p24 hybridoma cells are gained
方法：基因工程p24抗原免疫小鼠4次，历时2个月，取脾细胞与骨髓瘤细胞株sp2 0 ，用peg融合， hat选择培养和间接elisa筛选分泌抗p24抗体阳性的杂交瘤细胞，三次克隆化后得稳定分泌抗p24抗体的杂交瘤细胞株。 |
