| 1. | These are inserted into a cloning vector .
它们被插入克隆载体。 |
| 2. | An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1. 3 .
图13列出了引出一段外源DNA进入克隆载体的大概步骤。 |
| 3. | Construction of clone and expression vector of gene of vibrio parahaemolyticus
基因克隆载体和表达载体的构建 |
| 4. | An outline of the procedure for introducing a piece of foreign dna into a cloning vector is shown in figure 1 . 3
图1 3列出了引出一段外源dna进入克隆载体的大概步骤。 |
| 5. | In genetic engneering , nonviral dna can be inserted into a phage , which is then used as a cloning vector
在基因工程中，没有病毒的dna可以被插入到噬菌体中，用作克隆载体。 |
| 6. | The product of pcr named vp6 is approximate 1 . 3kb in length . the vp6 gene was cloned into pmd18 - t vector and sequenced
将其插入克隆载体质粒pmd18 - t的ecorv酶切位点处，构建重组质粒pmd18 - t ? vp6 。 |
| 7. | Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants , it has been used as a cloning vector in gnetic engineering
在许多双子叶植物中质粒在寄主细胞中是独立的复制单元，所以可以在基因工程中用作克隆载体。 |
| 8. | Recombinant cloning vector of pgem - hc and pgem - ha were constructed by ligating the hc and ha genes into pgem t east vector and transformating into jm109 . 3
分别将目的基因hypoderminc和hypodermina与克隆载体( pgemteasy )连接并转化到宿主菌jm109中，构建了重组克隆载体pgem - hc和pgem - ha 。 |
| 9. | A 358 bp pol ft cdna fragment framed with introduced restrict sites of xho i ( upstream ) and nhe i ( downstream ) was amplified by the method of rt - pcr , and then cloned into a ta - vector
Rt一pcr扩增得到358hp的pcilpcdna片段，并在其上下游引物中分别引人了h 。 i和ie内切酶位点。 l此片段克隆人ta克隆载体后，经h 。 |
| 10. | In this study , two kinds of specific promoter of b . thuringiensis : cry 3a promoter and btl - btll promoter were chosen to construct fusion genes to drive the expression of gfp gene in b . thuringiensis strain
本研究在构建苏云金芽胞杆菌质粒克隆载体pbmbi105的基础上，克隆了菌株ybt一1520的质粒，获得6 . 6kb的dna片段。 |
