| 1. | Spf chicken - indirect immunofluorescent assay
Spf鸡间接免疫荧光试验 |
| 2. | Laboratory animal - immunofluorescence assay
实验动物免疫荧光试验 |
| 3. | Indirect immunofluorescent antibody technique for epidemic hemorrhagic fever of rats
大鼠流行性出血热间接免疫荧光试验 |
| 4. | Protocol of indirect immunofluorescence assay for porcine reproductive and respiratory syndrome
猪繁殖和呼吸综合征.间接免疫荧光试验 |
| 5. | We used gv and m ii oocytes to detect the exist of jak2 in nuclear via immunofluorescence . the result showed that jak2 presents abundantly in the nuclear of mouse oocyte
采取完全生长的gv期和m期的小鼠卵母细胞，以间接免疫荧光试验检测发现， jak2大量存在于细胞核中。 |
| 6. | The recombinant meq - baculovirus was obtained by co - transfecting the insect sf9 cells with pblubac4 - meq and linearised bac - n - blue dna . the recombinant baculovirus was selected by plaque assay and confirmed by pcr technique and sequencing of the inserted gene
应用重组痘病毒表达的meq制备的单抗23b46对重组杆状病毒感染的sfg细胞及其裂解物分别进行间接免疫荧光试验、 westemblot和免疫沉淀试验的检测。 |
| 7. | The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination , results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm . by indirect fluorescent antibody test mdj - 01 strain was identified with tbev . a part of region encoding e protein was expanded by rt - pcr and sequenced . the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10 , respectively , which were 95 % , 94 % . mdj - 01 strain was identified with tbev again
应用间接免疫荧光试验进行血清学鉴定，结果表明mdj - 01株为tbev 。通过rt - pcr技术扩增部分e蛋白序列并测序，在genbank上进行同源性比较，发现mdj - 01株和森张株与tbevoshima5 - 10株的同源性最高，分别为94 、 95 ，从分子生物学水平上进一步证明mdj - 01株病毒为tbev 。在鉴定的基础上，本实验对两株病毒进行了核苷酸全序列测定。 |
| 8. | Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠，然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ，进行其分泌抗meq单克隆抗体( mcab )能力的筛选。 |
