| 1. | The pgl29 cdna is 1698bp containing an orf of 783bp encoding a protein with 260aa
Pelfq的全长cdna序列为1698hp ，含有一个编码260aa的orf ，编码蛋白的分子量为28 |
| 2. | Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase
以线性化的全长cdna为模板，体外转录得到了csfv基因组rna 。 |
| 3. | To obtain full - length lzp3 cdna , a pair of primers was designed according to the assembled lzp3 cdna sequence
根据已拼接出的lzp3全长cdna序列设计基因特异性引物，利用rt - pcr方法扩增lzp3全长cdna 。 |
| 4. | The full - length cdna of ttom1 , 1280 bp in length , encodes a deduced 288 a . a . protein with 7 - pass explasmic domains
Ttom1全长cdna有1280个碱基对，编码288个氨基酸，有7个跨膜区，是个典型的跨膜蛋白。 |
| 5. | Artificially using t - a cloning techniques and initiated one - step ligation of multi - fragments method were the main factors in the process of full - length cdna construction
T - a克隆的巧妙运用和多片段一步连接法的成功运用是全长cdna得以快速构建的主要原因。 |
| 6. | It would contribute to understanding the regulation mechanism of pollen tube growth only when more detailed studies of the biochemistry and physiological functions of ck2 had been done in pollen
至今，还没有人在花粉中检测ck2的存在以及研究它的功能。百合花粉ck2全长cdna的克隆，将有助于研究它在花粉中的生理生化功能。 |
| 7. | We detailed here a novel gene clsp , isolated from the dc cdna library by large - scale random sequencing . full length of clsp contains 3345 bp , with an orf of 1464 bp
经大规模随机测序和同源比较，我们从人树突状细胞cdna文库中分离到一个新表达序列标签( est ) ，其全长cdna为3345bp ，含有1464bp的开放读框，编码含487个氨基酸的蛋白。 |
| 8. | Finally , the full length cdna of real differential fragments were cloned by performing both 5 ' - and 3 ' - rapid amplification of cdna ends ( race ) and then the functional screening was performed by transferring the full length cdna into arabidopsis thaliana or nicotiana tabacum plant
108号克隆尚未做测序分析。通过race技术对152和233两个差异片段进行全长cdna克隆，获得了152的全长cdna序列和233的3 ’ 。 cdna序列。 |
| 9. | The deduced amino acid sequence of p1a5 was most homologous to the lipid transfer protein 3 precursor isolated from upland cotton , the lipid transfer protein sdi - 9 isolated from common sunflower , and the nonspecific lipid - transfer protein precursor allergen pru av3 isolated from sweet cherry
对得到的该片段分析表明，它是一种脂肪转移蛋白（ ltp ）基因的部分序列。采用5 ’端race技术获得了该基因的全长cdna序列n 。 |
| 10. | In this study , by combining rt - pcr and race , a full - length cdna encoding the lagurus lagurus zp3 ( lzp3 ) was isolated from the lagurus ' s ovary and cloned into a prokaryotic expression vector and a eukaryotic expression vector for further immunocontraceptive investigations
本论文研究工作采用rt - pcr与race相结合的方法，首次克隆了草原兔尾鼠zp3全长cdna并进行了序列分析，构建了真核表达质粒，为草原兔尾鼠免疫不育的研究奠定了基础。 |
