| 1. | In y2h , rapgap from c . elegans was used as bait to screen c . elegans cdna library . after - lth screen , 63 colonies were checked
在酵母双杂交系统中，将ppc97 rpg ”与ppc86七dna文库共转化入酵母菌y190细胞，经过一lth筛选后，有63个菌落拟为阳性菌落。 |
| 2. | Multi - genes co - transformation is very important for studying on metabolic pathway which were controlling by multi - genes and generation maker - free transgenic plants
摘要实现多基因在同一受体植株中的共转化在研究植物多基因控制的代谢途径和获得去选择标记转基因植株中具有巨大的应用前景。 |
| 3. | This article summarized the advance of the co - transformation research in recent years , especially expatiated the new types , mechanism , effect factors and application foreground of agrobacterium mediated co - transformation systems
笔者综述了目前国内外在共转化上的研究进展，尤其对农杆菌介导的共转化系统的新类型、作用机理、影响因素及其应用前景进行了阐述。 |
| 4. | When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a , 109 candidates interacting with nifa had been selected by testing for the expression of the his3 , ade2 and lacz reporter genes
诱饵质粒和文库质粒共转化酿酒酵母( saccharomycescerevisiae ) pj69 - 4a ，通过检测报告基因his3 、 ade2及lacz的表达进行筛选，初筛得到109个阳性酵母菌落。 |
| 5. | 11 interacting candidates proved to be the positives after isolating the mixed plasmids from yeast and recotransforming each purified ad / library plasmid and pgbd - nifa into s . cerevisiae pj69 - 4a respectively . the genes of the 11 positives had been sequenced and 4 different gene fragments were obtained
分别对其中的ad / library质粒进行分离纯化，将每个纯化质粒再次与诱饵质粒共转化酵母pj69 - 4a进行验证，得到11个阳性克隆，测序结果显示其中有4个不同的基因片段，分别命名为s4 、 s35 、 s64和s65 。 |
| 6. | Pebe2 vector containing the target gene and 35sih3 vector containing bar gene were co - bombarded into several indica rice lines shuhui527 , minghui86 and 612s etc . resistant seedling were obtained finally . in order to establish rice mutation library , agrobacteriwn vector pshk22 containing bar gene as screening maker was transformed iniojaponica zhonghual 1
在水稻的遗传转化过程中，通过基因枪法将含有目的基因sbe2b的质粒载体pebe2和含有筛选标记基因bar的质粒载体35sih3共转化到蜀恢527 、明恢86 、 612s等籼稻品种中去。 |
| 7. | The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged , in contrast , the interaction was disappear when the reading frame of each positive had been changed . the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
为了进一步验证这些克隆的编码产物与nifa蛋白的相互作用，将它们与nifa基因互换载体后共转化酵母，仍然可以激活三个报告基因的表达，而阳性克隆移码表达的重组质粒和pgbd - nifa的共转化物则不能在选择性平板上生长。 |
| 8. | Abstract ： based on present plant gene engineering techniques , some strategies to develop transgenic crops expressing multiple genes by constructing a multigenes expression vector or a number of expression vectors and then making one or more transformations or co - transformation are discussed and the prospects are described as well in this paper
摘要本文探讨了在利用植物基因工程技术的基础上，通过构建多基因或者多个表达载体，进行一次、多次基因转化或共转化方法获得转多价基因作物的一些策略，并进行了展望。 |
| 9. | Based on present plant gene engineering techniques , some strategies to develop transgenic crops expressing multiple genes by constructing a multigenes expression vector or a number of expression vectors and then making one or more transformations or co - transformation are discussed and the prospects are described as well in this paper
本文探讨了在利用植物基因工程技术的基础上，通过构建多基因或者多个表达载体，进行一次、多次基因转化或共转化方法获得转多价基因作物的一些策略，并进行了展望。 |
| 10. | According to above consideration , experiments was carried out as below : first , targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology . secondly , the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa , then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此，本试验首先扩增出整合在酵母基因组里的人血清白蛋白( hsa )基因作为目的基因，并将人血清白蛋白基因插入到一个含有人-乳白蛋白yac同源序列的重组型质粒载体，以构建整合型载体，再与另一个带筛选基因的质粒共转化入含人-乳白蛋白yac的酵母细胞体内。 |
