| 1. | Cultivation optimization of prolyl endopeptidase and high cell density fermentation
脯氨酰内肽酶培养条件的优化及高密度发酵 |
| 2. | 2 . the optimum temperature of eps is 37 ' c in petal and leaf of " samantha " and " belami "
两个品种切花花瓣、叶片中，内肽酶最适温度均为37 。 |
| 3. | Relationship between polyamines and ethylene evolution of cut rose flowers during senescence
两个切花月季品种花朵开放和衰老对乙烯的反应及其与内肽酶的关联 |
| 4. | 3 . the changes of ep activities of petal are more complicated than those of leaf during vasing
在瓶插期叶片内肽酶活性变化平缓，花瓣内肽酶活性变化复杂。 |
| 5. | 6 . there are very close relations between the rapidly decreasing of the soluble protein content , the sweetly enhancing of ep activities , the quckly increasing of fre
切花瓶插期间，花瓣、叶片中可溶性蛋白质含量迅速降低、内肽酶活性迅速增强、游离氨基酸含量迅速升高与切花衰老密切相关，可以作为切花开始衰老的生理指标。 |
| 6. | In order to seek the probable reasons " of difference of the process of opening to senescence between " samantha " and " belami " , the relations between senescence and ep were probed . the flowing results were obtained : 1
探讨了切花月季开花进程不同的品种瓶插期衰老与内肽酶的关系，以寻找引起它们开花进程不同的可能原因，主要得出了以下结果。 |
| 7. | From beginning to end , the ep activities of the optimum ph 10 of " samatha " petal is higher by far than added values those of the optimum ph 6 and the optimum ph 10 of the " belami " petal . this is one of likely reasons that " samatha " can open quickly and fully during vasing
整个过程中‘萨蔓莎’花瓣最适ph10条件下的内肽酶活性远大于‘贝拉米’最适ph6 、 ph10条件下的内肽酶活性的叠加值，这可能是‘萨蔓莎’瓶插期花朵能充分迅速绽开的原因之一。 |
| 8. | There are at least three kinds of ep in petal : thiol - proteinase , metallo - proteinase , serine proteinase . they gather at the site between 43 kd and 66 . 2 kd ( about 52 kd ) on the substrate gel electrophoresis . the optimum ph of thiol - proteinase , metallo - proteinase is ph 6 , which are looked as acid ep , the serine proteinase has two forms , one is acid ep ( ph 6 ) , and the other is alkali ( ph 10 )
花瓣中都至少有3种内肽酶：巯基蛋白酶、金属蛋白酶、丝氨酸蛋白酶，三者聚集在底物胶电泳胶条的43kd 66 . 2kd之间的位置，约为52kd ；前两种最适ph为6 ，是酸性内肽酶，后一种有酸( ph6 ) 、碱( ph10 )两种存在形式，既是酸性内肽酶又是碱性内肽酶。 |
| 9. | The content of soluble protein is also increased after deficit water stress , but the increasing of " belami " is more significant than that of " samantha " . 5 . after deficit water stress , in " samantha ' petals , the metallo - proteinase activities of the optimum ph 6 and the serine proteinase activities of the optimum ph 10 are increased markedly , the thiol - proteinase activities of the optimum ph 6 is decreased markedly
失水胁迫后显著提高了‘萨蔓莎’花瓣中最适ph6条件下金属蛋白酶、最适月季（ rosahybrde ）切花瓶插期间内肽酶与衰老的关系及失水胁迫对其影响的研究ph条件下的丝氨酸蛋白酶的活性，显著降低了最适ph6条件下琉基蛋白酶的活性。 |
| 10. | The changes of these endopeptidase are different during the former vasing of " samatha " and " belami " . the main eps in petals of " samatha " are the acid thiol - proteinase , metallo - proteinase and the alkali serine proteinase and the main eps in petals of " belami " are the acid thiol - proteinase and serine proteinase . during the later vasing the acid metallo - proteinase and the alkali serine proteinase become main eps in petals of " samatha " and " belami "
这3种内肽酶在两种切花瓶插初期(盛开以前)的表现不同： ‘萨蔓莎’花瓣中以酸性金属蛋白酶、巯基蛋白酶和碱性丝氨酸蛋白酶起主要作用， ‘贝拉米’花瓣以酸性巯基蛋白酶、丝氨酸蛋白酶起主要作用，后期‘萨蔓莎’和‘贝拉米’花瓣均以酸性金属蛋白酶、碱性丝氨酸蛋白酶起主要作用。 |
