| 1. | Southern blot of 4 green plants confirmed stable integration of tpsl gene into wheat genome
其中4株再生苗扩增出1500bp的tps1基因片段。 |
| 2. | Pcr analysis and southern blotting all confirmed that the ced - 9 gene has been integrated into the chromosomal dna of these transgenic plants
对所得到96株再生苗进行pcr检测，结果表明，阳性苗比例为34 。 |
| 3. | The coding region of the cdna of the phytoene desaturase gene was cloned by pcr and inserted into vector pbi121 containing intron - kanamycin
再生苗经过戍r和pcrs 。 : : hernbl 。 t分子检测证明获得了烟草转基因植株。 |
| 4. | The physical and chemical property of enolase and the predicted three dimensional strucure revealed that dseno protein was well matched to identified enolase
采用pcr 、 southern杂交、 northern杂交对转基因再生苗进行了分子生物学鉴定。 |
| 5. | To make the pcr primer that have adequacy enzyme sit flowed the sequence , ligate with the pgem - t easy after pcr . make sure the sequence by sequencing
经组织培养后，以pcr和rt - pcr法检测再生烟草，共得到转synnhap基因烟草4株， pbi121空载体浸染的对照烟草再生苗5株。 |
| 6. | Pcr analysis showed that approximate 9 % tested plants produced the target band . a few of the 9 % plants produced the band in southern blot analysis . additionally , the number of inserted copies of dsg 10 was different in different transgenic plants
结果显示有9的再生苗为pcr阳性，对pcr检测阳性苗进行southem杂交分析，发现dsg10已整合入烟草的基因组中，而且在这些苗中dsg10片段整合的拷贝数不同，部分pcr阳性苗southern杂交结果却是阴性。 |
| 7. | Four green plants were regenerated form resistant calli of wen . 6 derived form 1500 implanted embryos . no green plant was regenerated form calli of 200 non - transformed embryos . pcr assays of 4 green plants showed that two of them attained the expected size of amplified dna fragment ( 1500bp )
在含有20 - 60mg / l潮霉素的ms培养基上经诱导和继代培养后，获得了一批抗性愈伤组织，经过分化培养获得4株再生苗，对照的200枚幼胚未获得再生苗，对再生苗进行pcr检测。 |
| 8. | To make the ti plasmid pbi121 - nhap for transformants the tobacco ( nc89 ) , at the same time to make the pemu - mcs - n - nhap and pwubi . tml - nhap for transformants the yan wheat 18 . we getted 4 transgenic tobaccos , measured the ability of salt tolerance by treatment in 150 mmol / 1 nacl , found that the transgenic tobaccoes can growth and the control tobaccos ca n ' t
通过比较转synnhap基因烟草与对照烟草的耐盐性，发现4株转synnhap基因的烟草再生苗在含150mmnacl的培养液中处理两周后，植株外观无明显变化，叶片生长情况正常，而对照植株的生长则受到抑制，叶片明显萎蔫、失绿并最终脱落。 |
| 9. | T . identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis . both analysis the target bands were observed . so the integration of the cpti gene into mustard genome dna was confirmed . the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants
转基因芥菜植株的鉴定取再生苗的叶片提取dna ，进行pcr扩增和pcrsouthernblot分析，转化再生植株大部分呈阳性，而非转化的再生植株均为阴性，证明cpt基因已存在于芥菜基因组中。 |
