English translation for "剪接位点"
|
- splice site
splicing site
Related Translations:
剪接: montage; cut or edit a film; film editing 自剪接: rna ribosomal self-splicing 剪接体: spliceosomesplicesome
- Example Sentences:
| 1. | A new method for the splicing - site recognition of rice dna sequences was designed . based on the gt - ag intron organization principal , support vector machines ( svm ) was used to predict the splicing sites . through machine learning , a model was built on some test data set of true and pseudo splicing sites 根据真核生物内含子在剪切位点前后存在保守碱基的特征,用支持向量机技术构建分类器模型,有效地在基因组序列中识别剪接位点, 3位点识别的准确度87 . 96 ,在5位点识别的准确度达85 . 41 。 | | 2. | The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action , thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained . in this study , we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template . the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons 本研究设计以c tamrna为模板的反义cdna片段,从c ta基因5 ’端第94位到500位核苷酸段设计引物,目的片段407bp ,覆盖第116和188位两个aug密码子,也包含了第一外显子和第二外显子间的剪接位点:用常规分子生物学方法构建了反义片段的腺病毒表达载体( padeasy - 1系统) ;腺病毒载体经hek293细胞包装产生含反义片段的重组腺病毒,用氯化铯密度梯度离心法获得纯化的高滴度腺病毒;进行体外基因转移,分别用反义片段真核表达载体转染p388d1细胞和用重组腺病毒感染hela细胞,观察导入的c ta基因反义rna抑制细胞内组成型或诱导型c ta基因表达的作用,从而达到调控mhc -类分子表达的目的。 |
- Similar Words:
- "剪接前导序列" English translation, "剪接师" English translation, "剪接受体" English translation, "剪接受体,切拼受体,拼接受体" English translation, "剪接体" English translation, "剪接因子" English translation, "剪接员" English translation, "剪接作用" English translation, "剪截机" English translation, "剪节" English translation
|
|
|