Test method for semi - quantitative micro determination of acid number of used lubricating oils 废润滑油酸值半定量法微观测定方法
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We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively . at same time the differentiated cell were identified by immunocytochemistry method . results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu 以pdgf 、 t _ 3对nsc进行诱导分化,并分别用rt - pcr半定量法、 elisa法检测nsc在不同诱导因子作用下分化前后thr各亚型在转录与翻译水平上的表达变化,用免疫细胞化学方法鉴定分化后的细胞类型。