5 - ht induced local ca 2 + transients ( ca2 + sparks ) in cultured rat stomach fundus smooth muscle cells ( sfsmc ) was first observed by using the new fluorescent ca2 + indicator stdin and laser scanning confocal microscope . the mechanisms of initiation of ca2 + sparks and propagating ca2 + waves and their relation to e - c coupling were also discussed . 4 . the effects of reactive oxygen species on the spectral properties of the new fluorescent ca2 + indicator stdin were evaluated using four different oxidant - generating systems 应用于5一ht诱导胃底平滑肌细胞胞内游离ca2 +动员的机制研究，发现5一ht通过促进外钙内流和内钙释放使[ caz + j升高;在胃底平滑肌5一ht升高ca ， ‘ ，系通过5一htz受体介导的1p3 / ca ， ‘和dg / pkc双信使途径; 5一ht通过l型钙通道促进外钙内流。
