Rnai ( rna interference ) is a process by which double - stranded rna ( dsrna ) specifically suppresses the expression of a target mrna . since fire et . al Rnai ( rnainterference )是双链rna介导的、序列特异的同源基因转录后的沉默效应。
3.
Rnase p from e . coli is a ribonucleoprotein complex responsible for the 5 " maturation of trnas . the smallest substrate contains two important parts : ncca - 3 " terminal and rna helix area Rnasep是结构识别酶, m1rna底物至少包括两个要件: 1游离ncca - 3末端; 2特异互补的双链rna区域。
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Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china . cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively . total rna extracted from field samples were used for rna dot - hybridization 用侵染马蹄莲的dsmv3末端序列和黄瓜花叶病毒( cmv )的亚基因组启动子区互补dna序列为标记探针,对自然感病的天南星科植物进行rna斑点杂交,并结合双链rna分析、病毒提纯和形态学观察,对杭州等地16属天南星科植物的81个样品进行了病毒鉴定。