E . coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles . results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd 以构建的噬菌粒psurfpga转化具有琥珀突变的大肠杆菌xl1 - blue ，以辅助噬菌体m13k07超感染，进行青霉素g酰化酶基因的表达和在噬菌体表面的展示。
2.
A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein . the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis . it was demonstrated that constructed phagemid can still express penicillin g acylase 将包含信号肽和琥珀终止密码子uag ( amber )的完整巨大芽孢杆菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通过引入的11肽连接青霉素g酰化酶的c末端与噬菌体外壳蛋白gp3的n末端。
