English translation for "多次传代"
|
- high passage
Related Translations:
多次作案: repeatedly commit crimes 多次通过: multiple traversalmultiple-pass 多次覆盖: multifold coveragemultiple coveragemultiplecoverage 多次点火: multistage ignition 多次再生: multiple regeneration
- Example Sentences:
| 1. | In trpsin tolerance assay . this virus could resist to 1 % trpsis at 37 in an hour . in acid tolerance assay , this virus was resistant to ph3 . 0 and ph5 . 0 at 37 in 2 hours , and the average infection litre of the virus decreased little . in heat assay , at 50 , the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree . among these conditions , when at 50 in 30 minutes . the average infection litre of this virus decreased over 2 tilre . and when al 50 in an hour , cpe of ihis virus disappeared . when time was set for an hour . but with processed in different temperature as 50 60 70 , 80 , the virus losl the multiplication capacity complelely . in biological assay , we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory . then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second . with f81 cell line , after passaged for 12 times continuously with low concentration of fcs . the virus could produce cpe . however , with vero cell line . the virus could not procuce any cpe after many passages . the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig , fowl and cavy . by neutrolizaion assay , dxmv could be identified as a kind of ccv
理化学研究表明，该病毒为rna病毒，对氯仿、乙醚敏感；胰酶试验中，经37 、 1小时处理的病毒，仍然能够在猫源细胞fcwf细胞上生长，并且毒力基本保持不变；耐酸性试验中，病毒分别在ph5 . 0和ph3 . 0经37作用2小时，毒力仅下降一个滴度；耐热性试验中，该病毒在恒定温度50 ，设定不同时间，从5分钟到150分钟，毒力均有不同程度下降，其中， 50作用30分钟，病毒平均滴度下降2个单位； 50 ， 60分钟， cpe消失；恒定时间1小时，设定不同温度( 50 - 60 - 70 - 80 ) ，病毒在细胞上完全丧失增殖能力， cpe消失。生物学试验，利用实验室现有条件，选择不同的细胞系对该病毒进行培养，发现该病毒对猫源细胞fcwf最敏感； mdck细胞次之； f81细胞经多次传代，亦可出现cpe ；而vero细胞则不敏感。血凝试验表明，该病毒对猪、鸡、人及豚鼠的红细胞均无血凝性。 |
- Similar Words：
- "多次抽样检样" English translation, "多次出入境签证" English translation, "多次触球" English translation, "多次穿孔" English translation, "多次穿膜蛋白" English translation, "多次淬火" English translation, "多次存储卡" English translation, "多次打回" English translation, "多次打击" English translation, "多次代换编码系统" English translation
|
|
|
