English translation for "安普霉素"

apramycin

Example Sentences:

	1.	To study the biosynthetics genes of apramycin in streptomyces tenebrarius , the apramycin resistant gene was isolated by gunshot cloning firstly 为了研究黑暗链霉菌安普霉素生物合成基因，首先通过鸟枪克隆的方法克隆其安普霉素抗性基因。
	2.	The ligation mixture was used to transform s . lividans tk24 protoplasts . six colonies capable of growing in the presence of apramycin were isolated 连接液转化标准宿主菌s . lividanstk24原生质体，最终得到6个可以在安普霉素平板上生长的阳性克隆。
	3.	Amino acid feeding experiments were also performed in this study in order to investigate the pathway , by which nitrogen incoperated into apramycin molecule , and the regulatory mechanism of nitrogen metabolism 通过氨基酸添加实验研究了安普霉素生物合成途径中的氮的来源及调节机制。
	4.	Plasmid psyxl , containing a 12kb insert of s . tenebrarius dna , was isolated from one of six colonies . apramycin resistant gene was located in 1 . 5 - kb sphl - kpnl fragment of plasmid psyxl though further studies 阳性克隆中重组质粒psyx1含有12kb黑暗链霉菌的dna ，对12kb片段进一步分析将安普霉素抗性基因定位在1 . 5kbsphi - kpni片段上。
	5.	Streptomyces tenebrarius a04 , the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper . after treatment of spore suspension and mycelia shivered by supersonic with mutagen , combined with the application of screening models , some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23 , a2 - 30 , asm6 and al - 16 were obtained 本文以尼拉霉素单一组分?安普霉素产生菌s . tenebrariusa04 (发酵单位为2847u ml )为出发菌株，通过对单孢子悬液和超声波破碎菌体的诱变处理并复合筛选模型，获得了遗传特性稳定的单组分高产菌株： a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ，摇瓶发酵单位达4800 - 5200u ml 。
	6.	A 1 . 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e . coli / streptomyces shuttle vector with conjugation function ( containing orit gene ) . as a result of above procedures , a recombinant plasmid pid03 was obtained 将1 . 5kb的安普霉素抗性基因片段插入到aved基因中的nrui酶切位点，再将此灭活的aved基因片段插入到具有接合转移功能(含有orit基因)的链霉菌?大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pid03 。
	7.	Based on a 3 . 1kb pst i fragment of genomic dna of a wild s . avermitilis , a 1 . 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3 . 1 kb fragment , then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401 . competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively 以含有avec基因的3 . 1kb基因组dnapsti片段为基础，将1 . 5kb的安普霉素抗性基因片段插入到avec基因中的sphi酶切位点，再将此插入失活的avec基因片段连接到具有接合转移功能(含有orit基因)的链霉菌-大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pc05 。
	8.	In this study , the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering . insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins . when avec gene was inactivated by the same way , four " 1 " components were lost and only " 2 " components , the potential precursor of ivermectin , were accumulated 将该基因簇中的aved基因通过插入外源的安普霉素抗性基因片段使其失活，导致发酵产物中4个a组分(不需要的组分)的消失；将基因簇中的avec基因通过同样手段，使其失活，导致发酵产物中4个“ 1 ”组分的消失，而主要积累“ 2 ”组分(进一步改造可成为伊维菌素的前体b _ 2组分) 。
	9.	Arginine feeding experiment showed that nitrogen metabolism in the s . tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine , as a result , the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased . ( 2 ) arginine could be transformed into glutamic acid , so that the biosynthesis of apramycin was promoted 因而我们认为gln可能是安普霉素生物合成氮元素的供体。 arg添加实验结果表明， arg可能通过两种途径影响黑暗链霉菌体内的氮代谢： ( 1 ) arg可能影响胞外蛋白酶的活性，进而促进含氮大分子物质的分解代谢，补充发酵过程中的氮素来源。
	10.	The feeding of glutamic acid , glutamine and a - keto - glutaric acid respectively showed that glutamic acid and glutamine had no obvious effects on cell growth , but stimulated apramycin production greatly . with the same concentration of amino acids supplemented , glutamine showed a stronger stimulation effect than glutamic acid , while a - keto - glutaric acid showed a repression effect on apramycin production . it could be deduced from above results that glutamine possibly is the donor of nitrogen element for the biosynthesis of apramycin Glu 、 gln及?酮戊二酸添加实验结果表明glu 、 gln对菌体的生长无明显的影响，但能强烈促进安普霉素的生物合成，且相同浓度条件下gln的促进作用明显高于glu ，而?酮戊二酸则对安普霉素的生物合成有抑制作用。

Similar Words：

"安普纶" English translation, "安普罗利" English translation, "安普罗铵" English translation, "安普洛" English translation, "安普洛斯" English translation, "安普尼亚西" English translation, "安普生" English translation, "安普塔卡" English translation, "安普特希尔勋爵" English translation, "安普天润" English translation