English translation for "巢式pcr"
|
- nested pcr
Related Translations:
巢居: (of people) live on trees 巢菜: vetch; akta; common vetch 巢蕨: neot topteris nidusneottopteris nudius
- Example Sentences:
| 1. | Definition of nested pcr
巢式pcr的定义 | | 2. | Using nested pcr and draft human genome searching , we successfully cloned the tsarg2 and mouse homeobox gene and made rudiment study on its functions . there were three parts in the study , and the following is its main experimental methods and results
本研究利用巢式pcr技术和人类基因组草图搜索法，从上述est出发，于2000年6月成功地克隆了人类tsarg2基因及小鼠同源基因srg2 ，并对其功能进行了初步研究。 | | 3. | According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat , we designed primers and got the structure gene successfully . by 3 ' - race method combined with nested pcr , the 3 ' - end nuclear acid sequence was also obtained ; in additon , for the 5 ' - end sequence , we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer , and till now , partial 5 ' - end sequence has been amplified as well
本研究根据先前分离纯化所得天然tb22kda蛋白经maldi - tof - ms (质谱法)测得的氨基酸序列和文献报道的过敏蛋白核苷酸序列设计引物，扩增克隆了该过敏蛋白结构基因的编码序列；根据测得的序列设计特异性引物，并利用3 ’ - race方法结合巢式pcr扩增得到基因的3 ’末端；依据同源性比较的结果选用一段保守序列为5 ’引物，并根据结构基因内部序列设计3 ’特异性引物，进一步获得了该基因5 ’端的部分序列。 | | 4. | Part 1 : identification of a novel gene , tsarg2 , and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell , and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis . to rapidly attain human novel gene full - length cdna sequence , the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe , which was significantly changed in cryptorchidism and represents a novel gene . furthermore , a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分为三个部分，其主要实验方法及实验结果如下：第一章tsarg2基因的克隆与序列分析从已获得的在隐睾和正常睾丸对照中表达量有明显差异的est片段( be644542 )入手，设计了基因特异性引物和载体特异性引物进行巢式pcr扩增，结合人类基因组草图搜索法，从睾丸cdna文库中快速分离出人类睾丸凋亡相关基因的5末端而获得全长cdna ， genbank登录号为ay040204 ，同时应用生物信息学的方法克隆了该基因在小鼠中的同源基因， genbank登录号为af395083 。 |
- Similar Words：
- "巢曲菌素" English translation, "巢森" English translation, "巢珊瑚科" English translation, "巢山" English translation, "巢石海绵属" English translation, "巢式病例对照研究" English translation, "巢式二级减速齿轮" English translation, "巢式减速齿轮" English translation, "巢式减速齿轮箱" English translation, "巢式交配设计" English translation
|
|
|
