| 1. | According to the southern blot results , a 6 . 5 kb bamh i restriction fragment containing the gene coding for hal c8 was cloned and sequenced
Halcs的开放阅读框为849个核昔酸，编码283个氨基酸;它必须要剪切掉n端的197个氨基酸才能成为成熟的蛋白。 |
| 2. | The amplified cdna fragment was inserted to plasmid puc 18 . the sequencing result shows that length of cgh cdn a fragment is 669bp
正反向测序结果分析表明：克隆的草鱼生长激素基因cdna全长669bp ，开放阅读框( orf )包含633个碱基，编码210个氨基酸。 |
| 3. | The result of the agarose gel electrophoresis showed that the length of the full - length cdnas in the library was pooled mainly between 500 and 2 000 base pairs
结果表明获得的ejoi基因的cdna长度为876hp ，开放阅读框长度为759hp ，编码252个氨基酸。 |
| 4. | The full - length cdna sequence was finally generated by 5 ' race and 3 ' race respectively . at the same time , structure and function of ejol gene were primarily analyzed by bioinformatics method
结果表明获得的ejo3基因的cdna长度为1514hp ，开放阅读框长度为1368hp ，编码456个氨基酸。 |
| 5. | Open reading frames ( orf ) were found in some novel sequences from the library , after that , putative amino acid sequences were obtained , and hydrophobicity profile were analysed
我们还对部分序列做开放阅读框分析，利用推定的氨基酸序列进行疏水性分析，为研究蛋白的功能和细胞内定位提供线索。 |
| 6. | The complete human znf323 cdna sequence is 3179bp and contains a 1221 - bp open reading frame ( orf ) that encodes a 407 amino acid protein with a scan domain and 6 zinc finger c2i12 motifs
Znf323长3179个碱基，含有一个长为1221个碱基的开放阅读框，编码一个长为407个氨基酸的蛋白质，它包含一个scan框和6个c2h2型锌指。 |
| 7. | A careful comparison about the two ghrs was carried out as follows : scghr : the scghr cdna consists of 1958bp whose orf encodes a 602 aa protein , with a polyadnenylation signal and a ply ( a ) tail
南方鲇ghr ： scghrcdna全长1958bp ，包括一个编码602个氨基酸残基蛋白质的开放阅读框，一个加尾信号( aataa )和一个poly ( a )的尾巴。 |
| 8. | The sequence encodes an open reading frame of 518 amino acids . there is a transit peptide of 74 amino acids in the n terminal of the putative amino acids sequence coded by the cdna
该序列长为1758hp ，起始密码子位于84 86hp ，终止密码子为1658 166fop ，开放阅读框长为1557hp ，编码58个氨基酸序列，其中n末端含有74个氨基酸的转运肽。 |
| 9. | The sequence analysis showed that the sequence of il - 10 is identical to that published in genbank . its open reading frame is composed of 528 base pairs ( bp ) and predicted to code for a peptide of 175 amino acids
序列分析结果表明pil - 10基因与国外报道的基因序列完全一致，开放阅读框由528个核苷酸组成，推测编码的多肽由175个氨基酸组成。 |
| 10. | The positive cloned was sequenced by sanger " s dideoxy sequencing method . the results demonstrated the size of hn gene was 1930bp in length and included an open reading frame encoding a protein of 578 amino acid residues
经核苷酸测序，该基因全长1930bp ，含一个1734bp的开放阅读框，编码578个氨基酸，与国外报导的hn基因相比，核苷酸同源性为95 |
