| 1. | The bacterial cells are rendered competent .
使细菌细胞变成感受态。 |
| 2. | Cdna cloning and sequence analysis of human bactericidal permeability - increasing protein
自然感受态缺陷株蛋白质表达谱的初步分析 |
| 3. | 1 vertor , dh5a competent cells and long template expanded pcr kit were from invitrogen company
1载体， dh5a感受态细菌及pcr扩增试剂购自invitrogen公司。 |
| 4. | The positive clone was screened and identified by pcr and analysis of restriction endonuclease digestion
转化感受态大肠杆菌dh5 ，经pcr及限制性内切酶分析得到阳性克隆。 |
| 5. | Ligate the insert hbrp to a pgex - 5x - 1 expression vector . prepare eoli . bl21 competent cells
制备eoli . bl21感受态细胞，将pgex一hb即表达质粒，转化bl一21感受态细胞。 |
| 6. | The positive clone is selected by colony pcr and dna sequence is analyzed and determined by the m13 primer
Coltjm109感受态细胞，菌落pcr筛选鉴定阳性克隆，用m13通用引物进行dna序列测定。 |
| 7. | Subject find if the experiments in vitro can induce the competence of s . pn and explore whether competence plays a role in virulence
0的ctm中生长，另一组是感受态缺陷菌ph8 0的ctm中生长，把ph6 |
| 8. | Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
Pgem一t载体，感受态细菌jm109 ，反转录试剂盒和体外翻译试剂盒均购自promega公司。 |
| 9. | Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants
将cdna与载体连接，并导入dh5感受态细胞中，构建成cdna文库。 |
| 10. | Competence a characteristic of embryonic cells and meristematic cells whereby they have the potential to differentiate into any of several different types of cell
感受态：胚胎细胞和分生组织细胞具有的一种生理状态，它们可以具有分化为各种类型细胞的能力。 |
