| 1. | Cloning and sequence of mature peptiede of human bone morphogenetic protein7cdna
7成熟肽基因克隆及序列测定 |
| 2. | Expression of mature peptide of human bone morphogenetic protein - 7 in escherichia coli
7成熟肽在大肠杆菌中的高效表达 |
| 3. | Secreted expression of mature peptide gene of human bone morphogenic protein - 7 in pichia pastoris
7成熟肽基因在巴斯德毕氏酵母中的分泌表达 |
| 4. | Construction of recombinant pichia pastoris containing cdna encoding mature peptide of human bone morphgenetic protein - 7 bmp
7成熟肽毕赤酵母表达重组子的构建 |
| 5. | Large scale fermentation , expression and purification of recombinant human bone morphogenetic protein 4 mature peptide from escherichia coli
基因重组人骨形成蛋白4成熟肽在大肠杆菌中的大规模发酵表达及纯化 |
| 6. | There are no introns in all four genes and the length of the deduced mature bdnf ( 119 amino acid residues ) is the same
但是这4种动物bdnf基因编码区编码的成熟肽段长度一致，分别为含119个氨基酸残基的多肽。 |
| 7. | Fragment encoding the mature protein was used in this system . the expression of mature protein using this system was less effective compared with qiaexpress
后者表达n ?末端加gst的融合蛋白，用成熟肽编码片段进行了表达，发现表达量不及qiaexpress ( |
| 8. | Based on the high homologous n - terminal amino acid sequence between ba - dfe and subtilisin bpn , the fragment encoding ba - dfe mature peptide was amplified from the total dna of b . amyloliquefaciens dc - 4 by pcr
根据ba - dfe的n -端序列与subtilisinbpn高度同源设计引物， pcr扩增出ba - dfe成熟肽编码区片段。 |
| 9. | The coding sequences for the ntfs were then cloned into the expression vector pgex - 4t - 3 under the control of a tac promoter , and expressed in escherichia coli after induction with eptg
本论文经引物设计与合成后，利用pcr技术分别自大熊猫和朱鹤的基因组中克隆了gdnf基因和nts基因家族各成员成熟肽的完整编码序列。 |
| 10. | The cleavage site of putative signal peptide was predicted to occur between 28 ( ala ) and 29 ( ala ) thus the putative mature form of the protein composed of 92 amino acids with a molecular weight of 9 . 2 kda
推测的最可能的信号肽切割位点位于第28 （ alal和29 （ alal个氨基酸之间。椎测的成熟肽共有92个氨基酸，分子量9 zkda 。 |
