| 1. | 53 extracellular protease mutants were screened from the library
获得tn5gusa5插入突变体17820个，从中筛选胞外蛋白酶突变体。 |
| 2. | The strategies of large - scale mutagenesis and gene screening include chemical mutagenesis , insertional mutagenesis and gene trap
摘要用于大规模基因突变与筛选的主要策略有化学诱变、插入突变、基因诱捕。 |
| 3. | A single step transformation system for the generation of a large - scale t - dna insertional mutant population of rice was developed
基于水稻大规模t - dna插入突变体库的建立，我们发展了一个简单、快速、高效的农杆菌介导的一步转化系统。 |
| 4. | Insertional mutagenesis is a method for identifying genes by using the integration of dna as the mutagen , thereby facilitating the cloning of the mutated gene
插入突变是一种通过外源dna整合的方式来获得突变体，并克隆得到对应突变基因的方法。 |
| 5. | Two rice mutants ( named osddl and osdd2 ) , delayed in development for about one month were isolated by screening a rice insertional pool generated with ac / ds transposone system derived from maize
利用玉米ac ds系统构建水稻插入突变体库，从突变体库中筛选得到2株发育迟缓的突变体(命名为osdd1和osdd2 ) 。 |
| 6. | The use of retrovirus - mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth
运用反转录病毒介杂的插入突变技术，在脊椎动物斑马鱼中已经获得了许多影响胚胎发育和细胞生长过程的突变体，并找到了对应的基因。 |
| 7. | After amplifying genomic sequences flanked with tn5gusa5 by tail pcr and dna sequencing , then doing blast with genomic sequence of xcc 8004 , insertion sites of 10981 mutants were located precisely in xcc 8004 genome
经tail - pcr扩增tn5gusa5旁侧序列并测序，经与xcc8004基因组序列比对后获得精确定位的tn5gusa5插入突变体10981个。 |
| 8. | Two salt sensitive mutants were obtained and named as 042bm - x1 and 042bm - x2 . the test of salt tolerance showed that they cannot survive in the presence of 0 . 4mol / l and 0 . 5 mol / l nacl in solid and liquid fy medium , respectively
通过tn5 - 1063a突变得到042bm的转座子插入突变株，从3000多个突变株中筛选到盐敏感突变株042bm - x1和042bm - x2 。 |
| 9. | A mutant library of xanthomonas campestris pv . campestris ( hereafter xcc ) strain 8004 with 17820 clones was constructed by random transposon tn5gwsa5 mutagenesis , which cover 1800 predicted orfs of xcc genome
用转座子tn5gusa5诱变野油菜黄单胞菌( xanthomonascampestrispv . campestris以下简称xcc ) ，构建了xcc8004tn5gusa5插入突变体库。 |
| 10. | 4 . engineering dhqase ( arod ) - deficient e . coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e . coli chromosome . the mutant 31bk was engineered , in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e . coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system . the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene , so it improved carbon flow into the quinic acid biosynthesis direction
构建宿主菌基因精确定位突变株31bk ( arod : : arobkan ~ r )为了改变代谢途径脱氢奎尼酸( dhq )分支点上的代谢流量，使之充分流向目的产物奎尼酸合成方向，利用基因打靶技术构建了31884宿主菌arod基因精确定位插入突变体，使dhq脱水酶( dhqase )失活，阻断了碳代谢流流向芳香氨基酸生成的方向，同时用同源重组的方法将arob基因定位整合入染色体上，解除了限速酶对碳代谢流通过共同途径到达dhq的阻遏影响，并减轻代谢负担。 |
