| 1. | It is applicable to coinject two or three genes whose expression products have no antagonistic action . 4 . coinjection is a simple gene transfer method which saves time and money
非桔抗基因（转移基因的表达产物互不抑制）的共转移（显微注射法）是一种既经济、又有效的方法。 |
| 2. | Gene transfer is a new and widely used technology in bioscience field . at present over 80 percent of transgenic animal models are constructed by means of microinjection
转基因是近年来在生命科学领域广泛应用的新兴技术，目前80 %以上的转基因动物模型是利用显微注射法构建完成的。 |
| 3. | Gene transfer is a new and widely used technology in bioscience field . at present over 80 percent of transgenic animal models are constructed by means of microinjection
转基因是近年来在生命科学领域广泛应用的新兴技术，目前80以上的转基因动物模型是利用显微注射法构建完成的。 |
| 4. | Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection . the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours . the fluorescent ratio of gastrula embryo period was up to 25 %
采用显微注射法将这种重组基因转化1细胞期的金鱼受精卵，实验结果如下： ( 1 )显微注射后，根据胚胎发育分期，胚胎在显微注射后36小时开始能在紫外灯下观察到荧光，原肠期发荧光的胚胎比例为25 ，后期发育荧光率逐渐下降，肌肉效应期后又相对稳定。 |
| 5. | They were coinjected into the male prenuclei of fertilized eggs with hsa . dna together . after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings , the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection
用显微注射法分批把这三种不同的dna片段导人小鼠受精卵雄原核，并移植入假孕受体鼠，产生的65只n代小鼠中，经pcr和southern杂交检测，表明转入的基因在12只小鼠体内有整合段。 |
| 6. | They cannot naturally produce essential fatty acids - beneficial nutrients found mainly in plant and fish oil , so they must rely on a dietary supply . here we want to establish transgenic mice carry a tissue - specific expression gossypium hirsutum - 6 fatty acid desaturase ( fad2 ) gene which can add a double bond into an monosaturated fatty - acid hydrocarbon chain and convert oleic acid to linoleic
本试验试图构建一个肌肉组织特性的- 6脂肪酸去饱和酶(脱氢酶)真核表达载体，通过原核显微注射法把棉花的- 6脂肪酸去饱和酶导入到小鼠体内，以小鼠为动物模型，建立动物体内的必需脂肪酸合成代谢通路。 |
| 7. | The recombination gene micyoinjected is 5nl dna ( about 106 - 107 ) into everyone oocytes or cytoplasm of grass carp . there were 32197 oocytes of 12 series via micyoinjection and achived 12945 fries in 2000 year , by hatching and raising . genomic dna was extracted from grass carp who were transformated in 1120 grass carp presented postive reaction by blood
实验通过将hu - - ifn基因重组分子采用显微注射法将这种重组基因转化草鱼1 - 2细胞期的受精卵，即以narashige显微注射仪，将5nldna (约10 ~ 6 10 ~ 7分子)直接注射到选择好的受精卵核的动物极上。 |
