| 1. | It was demonstrated that the enzyme has 43 . 7 % a - helix , 26 . 9 % - pleated , 29 . 4 % random coil by the analysis of circular dichroism spectrum
通过动力学方法测得该酶最适ph为8 . 0 ，最适温度为30 ，并对热不稳定。 |
| 2. | Both the two phytase had a optimal temperature and ph at 60 ? and ph 4 . 5 except the baculovirus derived phytase seemed to be more thermostable
两个表达系统中表达的重组appa植酸酶的最适温度与最适ph一致，分别为60与ph4 . 5 。 |
| 3. | 30 % ~ 60 % ammonium sulfate fractionation of the crude extract was carried out at 4 ? . the optimum temperature and ph for it were 37 ? and 6 . 5
结果发现，该酶作用的最适ph为6 . 5 ，最适温度为37 ；在ph5 8之间稳定，其热稳定性不高。 |
| 4. | There is at least a serine proteinase in leaf , it ' s optimum ph is ph 10 , it ' s molecular weight is about 52 kd
叶片至少有一种丝氨酸蛋白酶，最适ph为10 ，分子量约为52kd 。花瓣最适ph存在差异， ‘萨蔓莎’为ph10 ， ‘贝拉米’为ph6和ph10 。 |
| 5. | The optimal temperature , the optimal ph value and the optimal substrate ( l - aspartic acid ) concentration for the enzymatic reaction by adl were 40 , 5 . 0 and 66 . 5 ~ 133g / l , respectively
对固定化细胞性质进行的初步研究发现，其最适温度为40 ，最适ph为5 . 0 ，最适底物浓度范围为66 . 5 133g l 。 |
| 6. | While , in " belami " petals , the metallo - proteinase and the thiol - proteinase activities of the optimum ph 6 and the serine proteinase activities of the optimum ph 10 are also increased markedly
而在‘贝拉米’花瓣中最适ph6条件下金属蛋白酶、琉基蛋白酶和最适ph条件下丝氨酸蛋白酶的活性都显著提高了。 |
| 7. | It was very slow at 5 but become higher above 25 . the enzyme from abomasums used in cheese production was rough extracts containing chymosin and pepsin . through the gel filtration chromatography and ion exchange chromatogra
羔羊胃蛋白酶最适凝乳温度为45 ’ c ；在45处理30inin ，酶活性开始下降， 60c处理30lliln ，酶活性完全丧失；酶的最适ph为1 |
| 8. | 5 and was virtually inactive above ph 6 . 0 . when the enzyme was incubated at various ph values at 37 ? c for 30 min in the absence of substrate and the residual activity was measured , the phytase was found to be stable at the ph range of 2 to 8
该酶反应的最适ph值为5 . 5 ，在phz一6之间都有较高的酶活性， ph大于6时植酸酶的活性迅速下降，在phz一8之间的稳定性很好，活性> 70 % 。 |
| 9. | The enzyme is homologous dimmer with molecular mass 102600 as determined by gel filtration on hplc and subunit mass 52042 as determined by maldi - tof mass spectrometry . the optimal ph of d - hydantoinase is at near 9 . 5 and the optimal temperature is at 45
通过对其生化性质的研究表明，其单体m _ r为52042 ，天然m _ r为102600 ，最适ph为ph9 . 5 ，最适温度为45 ，不同的二价金属离子对酶活性有不同的影响。 |
| 10. | Preparing the lactase solution expressed in e . coli , we determinated the optimum ph , ph stability , optimum reaction tempreture , thermal stability , effect of metal ions and chemical reagent , km and vmax its optimum ph was 5 . 8 and 6 . 4 to 7 . 5 , and the optimum tempreture was 45 to 52
以onpg为底物，分别测定了其最适ph值及ph稳定性、最适温度及热稳定性、金属离子和化学试剂的影响以及k _ m值和v _ ( max )值。 |
