| 1. | Fibre reinforced plastics - determination of water absorption by immersion 纤维增强的塑料.用浸泡法测定塑料吸水性 |
| 2. | Plastic sheets . determination of dimensional variations after heating . immersion method 塑料薄板.受热后尺寸变化的测定.浸泡法 |
| 3. | Paints and varnishes . determination of resistance to liquids . part 2 : water immersion method 涂料和清漆.耐液体性测定.第2部分:水浸泡法 |
| 4. | Ship coatings - determination of resistance to salt water - salt water and hot salt water immersion method 船舶漆耐盐水性的测定盐水和热盐水浸泡法 |
| 5. | Semiconductor devices - mechanical and climatic test methods - rapid change of temperature - two - fluid - bath method 半导体装置.机械和气候试验方法.温度速变.双流体浸泡法 |
| 6. | And the traditional immersion method and gbj82 - 85 are applied less due to the long test period , heavy and lower efficiency respectively 传统的氯离子渗透浸泡法测试周期较长,而国标gbj82 - 85方法测试工作繁重、效率很低,所以这两种方法也很少被采用。 |
| 7. | In the study of floral - dip method , flowering inflorescences of oilseed rape growing in field are immersed in floral - dip solution resuspended with cultured agrobacterium tumefaciens lba4404 or eha105 , resided binary plasmid pbi121 在油菜花序浸泡法( noraldip )转基因的研究中,用含双元载体及报告基因的农杆菌在生产大田直接浸染正在开放的油菜花朵。 |
| 8. | The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated . the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one . the cocultivation time is 28 days . the experiment shows that : the erniuxin cotyledon regeneration frequency is very high , and that the dongnong901 hypocotyl regeneration frequency is very high 确立了农杆菌介导转化过程中基因型是影响转化率的主要因素。通过极差分析,基因型为“二牛心” ,菌液浓度15倍的,外植体为子叶,感染方法为浸泡法是最佳的组合。农杆菌与外植体共培养的时间为28h 。 |
| 9. | Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ) . nineteen individual kanamycin resistant plants were obtained . t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l ) 将ssvp和ssmapkk的全长cdna分别克隆入植物表达载体pcambia1300和prok中,导入根瘤农杆菌gv3101后,由花浸泡法进行拟南芥遗传转化,转化ssvp盐地碱蓬ssop和ssmapkk基因的克隆与功能鉴定的拟南芥在含潮霉素( 25mg )的ms培养基上筛选,获得t ;代转基因植株。 |
| 10. | The objectives of this study are to clone the ban fragment of brassica napus and to apply the floral - dip in the transformation of oilseed rape to establish a convenient method for oilseed rape breeding . ban is dfr - like gene in chalcone synthesis pathway functioned as a negative regulator , and has been well studied in arabidopsis thaliana 为利用转基因技术创造甘蓝型黄籽油菜,本文克隆了与甘蓝型油菜( brassicanapus )色素生物合成有关的ban基因同源片段,并探索了甘蓝型油菜花序浸泡法转基因新方法。 |