| 1. | Enolase isolated from peas was sensitive to fluoride .
从豌豆提取的烯醇酶对氟化物敏感。 |
| 2. | Dynamic changes of serum neuron - specific enolase level in patients with transient brain ischemic attack
短暂性脑缺血发作患者血清神经元特异性烯醇酶水平的动态变化 |
| 3. | Furthermore , its enolase gene , related to salt stress toleration , has been cloned and identified for its function
并且对盐藻耐盐相关基因? ?烯醇酶基因进行了克隆和转基因功能鉴定。 |
| 4. | Relationship of serum neuron - specific enolase level with neurologic impairment and prognosis in patients with acute cerebral infarction
急性脑梗死患者血清中神经元特异性烯醇酶含量与神经功能缺损及预后的关系 |
| 5. | Clinical significance of dynamic changes of plasma neuron - specific enolase , endothelin and calcitonin gene - related peptide in elderly patients with acute cerebral infarction
老年急性脑梗死患者血浆神经元特异性烯醇酶内皮素降钙素基因相关肽的变化 |
| 6. | A full - length enolase cdna of 1734bp from d . salina ( genebank accession no . 245549 ) was acquired by 5 " race and 3 " race . the sequence of enolase gene from d . salina ( dseno gene ) was analyzed by using bioinformadcs
选择盐藻23srna作为内标基因，利用半定量rl ，一pcr对经不同渗透震动后( o一48小时)烯醇酶基因转录水平的表达量进行了测定。 |
| 7. | The traditional homogene cloning method was modified on two aspects : first , homo - primers was designed at the sites with minimum degeneracy instead of maximum homology , secondly , some " n " bases were replaced with inosine in degenerate codes , resulting hi the degeneracy reduced from over 1000 times to below 100 times
改进后的同源引物简并度从1000倍以上降低至100倍以下，利用rt一pcr成功地扩增出一条630bp的盐藻cdna片段，该片段与其它物种的烯醇酶基因具有很高的相似性。 |
| 8. | The dna sequence had a complete orf ( open reading frame ) , which coded a protein of 479 amino acid residues . the protein sequence of enolase which contained the conserved domain , was homologue to enolase of other organisms . it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii
并将其中的一个gus基因用目的片段烯醇酶基因替换，构建了可以在植物中高效表达的载体pcambia2301g一enolase ，成功地将其转入根癌农杆菌eha105中，为下一步进行转基因植物的研究作准备。 |
