| 1. | With my porch and my pillow , my pretty purple petunias
那里有我的前廊我的枕头，我漂亮的紫色矮牵牛。 |
| 2. | We also studied factors effecting the variation of p . hybrida by tissue culture
并对矮牵牛叶片组织培养及产生变异的影响因素等进行探讨。 |
| 3. | Patted dry on the filter paper , transferred to ms3 medium and co - cultured for 3 - 4 days in darkness
用eha105 ( pbinc川+ )转化的纯白色矮牵牛，所得转化株系命名为wc “卜。 |
| 4. | The prescription of the tissue culture of petunie hybrida and the dna level of phenotypic variation seed lings produced by tissue were discussed
摘要探索了矮牵牛组织培养的配方，对组培过程中产生的表型变异植株进行了dna水平的分析。 |
| 5. | In this study , map2 and sap2 , two homologs ofap2 in arabidopsis and hap2 in hyacinthus orientalis l . , were isolated from the flower buds of apple ( mains pumila mill . )
拟南芥的ap2和ant基因、玉米的gl15 、 ids1基因、矮牵牛的phap2a 、 phap2b基因以及风信子的hap2基因都属于这一基因家族。 |
| 6. | The leisure and cultural services department ( lcsd ) presented the 2002 hong kong flower show at victoria park from 8 to 17 march with " hong kong in full blossom " as its theme and garden petunia as its theme flower
这是康乐及文化事务署第三年举办这个本港一年一度最大型的园艺盛事。今年展览主题是万紫千红喜迎宾，主题花则是矮牵牛。 |
| 7. | Flavonoid - 3 ' , 5 ' - hydroxylase ( f3 ' , 5 ' h ) corresponding to the genetic loci hfl and hf2 , a member of the cytochrome p450 family , is the key enzyme in the anthocyanin biosynthesis , which is generally required for blue or purple flowers
本研究以紫蓝色矮牵牛基因组dna为模板，利用pcr技术获得了两个扩增产物，分别将其克隆至pgem - teasyvector上，进行了全序列分析。 |
| 8. | Gene engineering technology is more superior than the cross breeding and directive breeding technology with its short cycle , low cost and high benefit . though traditional breeding technology has been used for a long time . now the direct reports for the changes of the flower color by the chi ( chalcone isomerase ) gene are a few what we known .
关于花色结构基因查尔酮异构酶chi ( chalconeisomerase )基因对花色改变的直接报道很少，因此，本论文选用了chi基因为目的基因，以纯深红色和纯白色矮牵牛( petuniahybidavilm . )为材料，研究了chi基因的共抑制和反义抑制以及表达产物增加对花色改良的作用，并在花色改变植株中首次观察到花器官变异。 |
| 9. | After co - cultivation , all the explants were washed in liquid mso medium containing 500mg / l cef for 2 hours . washed with sterile water for 5 times and patted dry on the filter paper again . subsequently , the explants were transferred to ms1 medium supplemented with 200mg / l cef for 3 - 6 days delay selection and then transferred to ms1 medium supplemented with 200mg / l cef and 50mg / l km for selection culture
对普通pcr检测两种结果均为阳性的转化株系，又同时以355和nptll为目的片段进行2xmpcr检测，结果得到tg一rch卜n二l一20 (表示检测结果为阳性的转基因矮牵牛rch卜有加个株系) 、 te一rch ，一n = z一10和to一wch ， + n = l一10 。 |
| 10. | Many studies show that leafy is high homolog even among distantly related plant species . exception of these , little studies on tissue culture and transformation of ginkgo have been done . this paper emphasizes on the isolation , cloning and analysing two ginkgo orthologs of leafy from the male tree
为此，本实验从银杏leafy同源基因的克隆入手，分析其雌雄株lfy基因结构差异，构建lfy基因的植物正义反义表达载体，建立矮牵牛遗传转化体系，以研究银杏lfy同源基因的功能，同时建立了银杏组织培养体系，为银杏的遗传转化和提早开花结果奠定基础。 |
