Css is the one of the colonization factor antigens which is a protective antigen can cause immune reaction by the means taking orally . in this study , carrot was separately transformed with agrobacterium tumefacience strain lba4404 that contains ctb or ctb - cs3 fused gene in order to get oral diarrhea vaccine . this is a potent strategy to produce etec oral vaccine 本研究期望通过农杆菌转化系统将ctb (霍乱毒素b亚基，可作为佐剂和载体)基因和ctb - cs3融合基因分别转入胡萝卜植株，使ctb和ctb ? cs3融合基因稳定整合到胡萝卜基因组内，希望以此获得以胡萝卜为受体的etec口服疫苗，使服食者在进食的同时就可获得腹泻免疫。
