It ' s meaningful to study the function of rab proteins in ciliates and further to explicit the mechanism of vesicular trafficking . the rob gene was amplified from macronuclear dna of euplotes octocarinatus . the size of gene was 783 bp long with an orf of 624 bp encoding eorabl protein and containing three in - frame tga codes 本研究利用pcr技术从游仆虫( euplotesoctocarinatus )大核dna中扩增出rab基因,并对该基因进行序列分析,该基因全长为783bp ,两端为端粒序列,编码框为624bp ,编码207个氨基酸,开放读框中有3个tga ,在此编码半胱氨酸。