| 1. | Detection of lily mottle virus by rt - pcr using special primers and degenerate primers
应用特异引物和简并引物检测百合斑驳病毒 |
| 2. | Automated programming of degenerate primers and the cloning of the diamondback esterase gene
程序化设计简并引物与克隆小菜蛾酯酶基因 |
| 3. | Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae . six novel rip gene fragments ( 408 ' bp ) , 1 from benincasa hispida and 5 from cucurbit a moschaia
根据葫芦科rip上、下游两段高度保守的氨基酸序列设计简并引物对ly1 ly2 ，对基因组dna进行pcr扩增，首次建立了rip新基因快速筛选体系。 |
| 4. | Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis
利用genbank中已经登录的其它植物中该酶基因的保守序列，设计一对简并引物，采用rt - pcr技术，从茶树扩增出208bp的cdna片段。 |
| 5. | These results revealed that halocin c8 is cytocidal and its primary target might be located in the cell wall of the sensitive cells . two degenerate oligoxynucleotide primers ( c8 - 2 , c8 - 4 ) were designed according to the n - terminal amino acid sequence of halocin c8
根据嗜盐菌素cs的n端氨基酸序列设计了一对简并引物( cs一2 ， cs礴) ，利用这对引物从as7002的总dna中扩增出halcs的部分序列。 |
| 6. | Three special bands as pcr products , obtained by using primer no . l , are reclaimed , and then used to cloning and sequencing . through homologous comparison with data from international nucleotide databank , the band ' s functions have been simply analyzed
对1号简并引物扩增出的3条特异性条带进行了回收、克隆和测序；与国际核苷酸数据库进行了同源性比较，对特异性片段的功能进行初步的分析和确定。 |
| 7. | To elucidate further the presense and function of integrin - like protein , we try to clone 3 " terminus seqence of integrin - like gene from germinated pollen of lily by 3 " race using degenerate primers corresponding to the conserved cytoplasmic regions of a and 3 subunits
而要进一步从分子水平上证实类整合素的存在以及研究它的功能，必须克隆其基因。根据动物整合素、亚基的胞质域保守序列设计简并引物，利用3 race的方法扩增、亚基的3末端序列。 |
| 8. | A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis . a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ) , which was used as a probe for screening tomato fruit ( pink stage ) cdna library . one positive clone containing entire coding region were isolated , which was identified as a new member of acbf family by blast server , and named as leacbf
根据genbank (中的拟南芥、烟草acbf家族成员序列比较的结果，在该基因的保守区设计简并引物( degenerateprimer ) ，以转色期普通番茄果实的rna为模板，进行rt - pcr扩增，获得239bp的扩增片段，以此片段作为探针筛选转色期普通番茄果实cdna噬菌体文库，获得了包含全长编码区的阳性克隆。 |
| 9. | Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease . serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography . the t profile , t stability profile , ph profile , substrate specificity and inhibitor were tested
该酶分子n -端的氨基酸序列为avidtgveashpef ，通过n -端氨基酸序列设计简并引物，提取被毛孢owvt - 1的总rna ， rt - pcr克隆了该酶的成熟肽编码基因( 1000bp ) ，序列分析表明，丝氨酸含量高达12以上。 |
| 10. | Then , a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer . combined with the opuaa - up , a 2 . 3 kb fragment was obtained through pcr . blast result showed a fragment which contained the partial opuaa , the whole opuab and partial opuac sequences were obtained
再次，根据甘氨酸甜菜碱atp转运系统底物结合蛋白的氨基酸保守序列设计下游简并引物，与atp结合蛋白的上游简并引物组合，经pcr扩增获得2 . 1kb的条带，测序后通过blast比较，结果显示获得atp结合蛋白基因的部分序列、通透酶的全部编码序列和部分甘氨酸甜菜碱结合蛋白基因的核苷酸序列。 |
