English translation for "类凝血酶"
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- batroxobin
Related Translations:
前凝血酶: prethrombinprothrombaseprothrombinthrombinogenthrombogen 凝血酶原激酶: factor xathrombokinase,thrombozymethrombozyme 凝血酶原时间: protimepro-time prothrombin timepro. timeprotrombin time
- Example Sentences:
| 1. | Cloning and expression of the cdna of a thrombin - like enzyme from the venom of gloydius shedaoensis
大连蛇岛蝮蛇类凝血酶基因克隆与表达研究 | | 2. | The recombinant gloshedobin binding to the affinity adsorbent con a indicated that it is a glycoprotein
此重组类凝血酶在37t中性溶液中保存过夜将分解成小肽，但在0t下很稳定。 | | 3. | Compared with gloshedobin , gussurobin has three different amino acid residues : gln66 vs . glu66 ; gly117 vs . asp117 ; thr 225 vs . he225
两种蛇毒类凝血酶的cdna序列及推导的氨基酸序列均为首次报道，并已申请了专利保护。 | | 4. | Its enzymatic activity showed less similarity with gloshedobin . therefore , these three residues " replacement may influence the stability of the advanced structure of the enzyme
这两类蛇毒类凝血酶基因的成功测序为其基因工程及其结构和功能的研究创造了条件。 | | 5. | The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask . having been induced with methanol for 36 hours , the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次将大连蛇岛赅蛇毒类凝血酶成熟基回克隆到表达载体ppicgk中，经电激转化至毕氏酵母菌株gs15中，再经甲醇诱导，在150ml摇瓶畔1获得细胞外分泌表达产物。 | | 6. | According to the purity and the activity recovery , the recombinant enzyme was well purified by both of these two separation combinations . like natural gloshedobin , the recombinant enzyme exhibited strong esterase activity using tripeptide p - nitroanilide derivatives as substrate , but hydrolyzed n a - p - tosyl - l - arginine methyl ester ( tame ) or n a - benzoyl - l - arginine ethyl ester ( baee ) very weakly
与天然蛇毒类凝血酶一致，当用三肽p山itroanilide衍生物作为底物时，分泌表达的重组大连蛇岛蝗蛇毒类凝血酶具有较强的生色底物活性，但用精氨酸甲酯如tame （ na厂tosyl l ar 。 | | 7. | Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ) , strain bl21 ( de3 ) , to study their expression in prokaryotic cell . the gene was expressed under t7 promoter with a fusion protein partner of thx . tag and a 6x his . tag at its n - terminal . having been induced by iptg for 4 hours , the recombinant enzyme was examined in the cytoplasm by sds - page analysis
将大连蛇岛蝮蛇和长白山白眉蝮蛇毒类凝血酶基因分别克隆到大肠杆菌表达载体pet - 32 ( a ) +中，在t7启动子下表达出融合蛋白，融合部分为硫氧还蛋白，位于类凝血酶基因上游，并在其n端带6xhistag标签以利于表达产物的分离纯化，经热激转化至宿主菌bl21 ( de3 )中， iptg诱导斗小时后收获菌体。 | | 8. | Its amino acid sequence exhibits significant homology with those of other thrombin - like snake venom enzymes . based on the homology , the catalytic residues and disulfide bridges of gloshedobin were deduced to be as follows : his43 , asp88 , ser182 and asp176 ; and disulfide bridges , cys7 - 141 , cys 28 - 44 , cys 76 - 234 , cys120 - 188 , cys152 - 167 and cys178 - 213
以同样的方法，得到长白山白眉蝮蛇( gloydiusussuriensis )毒类凝血酶基因，并比较了它与大连蛇岛蝮蛇类凝血酶基因序列的差别，发现两者的同源性高达98 ，只有三个氨基酸不同： gln ~ ( 66 ) |
- Similar Words：
- "类尿胆素" English translation, "类凝固素" English translation, "类凝集素" English translation, "类凝集素反应" English translation, "类凝胶" English translation, "类牛病毒性腹泻病源体" English translation, "类牛痘" English translation, "类牛皮癣" English translation, "类诺瓦克病毒" English translation, "类泡沫" English translation
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