The immunohistochemical method and western blot were preformed to show the expression of egfr and rt - pcr method was applied to determine the expression of egfr mrna in placenta trophoblast of pregnancy with pih . 3 . egfr of jar was determined by immunohistochemical method and egfrmrna was analyzed by rt - pcr method 电子显微镜观察正常妊娠及妊高征患者两组胎盘滋养细胞的超微结构,应用免疫组织化学方法及westernblot方法测定妊高征胎盘滋养层细胞表皮生长因子受体( egfr )的表达,以及rt一nr技术对egfrmrna进行定量分析。