| 1. | Optimum ph and temperature for the enzyme activity were approximately ph8 . 0 and 45 , respectively
对胆固醇氧化酶的酶学及物理化学性质进行了初步探索。 |
| 2. | As a excellent material for preparation of biomembrane , cellulose acetate ( ca ) is a fine carrier for the immobilization of cholesterol oxidase ( cod )
醋酸纤维素( ca )作为一种优异的生物传感用薄膜制备材料，是胆固醇氧化酶( cod )优良的固体载体。 |
| 3. | Different expression systems were constructed using different expression vector including pet - his and e . coli topi of " and bl21 host cells
利用pet - his等不同表达载体和大肠杆菌top10f 、 bl21 ( de3 ) plyss表达宿主菌，成功构建了胆固醇氧化酶基因的不同原核表达系统。 |
| 4. | Therefore , research on cholesterol oxidase has become more and more important . a 1 . 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi . 4 - 2g2
本研究采用pcr方法从分泌胆固醇氧化酶的马红球菌菌株4 ? 2g2基因组dna中，扩增出特异dna片段，该扩增片段全长约1 . 6kb 。 |
| 5. | Strain sa - coo by southern hybridization . a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357 , a streptomyces - e . coli bifunctional vector carrying two cohesive sites
为了获得胆固醇氧化酶基因，以大肠杆菌-链霉菌双功能柯斯质粒phz1357为载体，构建了灰色链霉菌atcc14811的基因组文库。 |
| 6. | This enzyme is industrially important and useful for the clinical determination of total or free serum cholesterol by coupling with related enzymes , the determination of the steric configuration of 3 3 - hydroxysteroids and the preparation of hormone
胆固醇氧化酶是胆固醇代谢的关键酶之一，它催化胆固醇氧化及异构反应，生成胆甾- 4 -烯- 3 -酮和过氧化氢。 |
| 7. | The 2 . 3kb fragment was then cloned to pbluescript ii sk ( + ) . the resulting plasmid , phz1754 , was subjected to progressive unidirectional deletions using exonucleaseiii provided in the erase - a - base system for dna sequencing
其后的亚克隆和功能试验将胆固醇氧化酶基因定位在phz1148的2 . 3kbecori - sal片段上，将该片段克隆到大肠杆菌载体pbluescript sk ( + )上，形成质粒phz1754 。 |
| 8. | Moreover , together with cholesterol esterase and catalyse , cholesterol oxidase can be used as test reagent to determine the total cholesterol content in blood plasma for the diagnosis of such as cardiovascular reflex and atherosclerosis clinically
另外，在临床上胆固醇氧化酶可以与胆固醇脂酶和过氧化氢酶一起作为血浆总胆固醇含量的检测试剂，用以诊断冠心病、动脉粥样硬化等心脑血管疾病。因此，对胆固醇氧化酶的研究越来越受到重视。 |
| 9. | Cholesterol oxidase is a type of protein that was found to have pest - resistant activity only years ago , its toxity to such sensitive pests as cotton boll weevil is euqivalent to previously found bt . endotoxins . the protein can also inhibit the growth and development of cotton boll worm
胆固醇氧化酶对其敏感害虫?鞘翅目的棉铃象甲的毒杀能力与早期发现的bt .毒蛋白相当，对鳞翅目的一些害虫如烟蚜夜蛾等也有较强的抑制其生长发育的作用。 |
| 10. | Sequence analysis showed that , this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi . after cloning and subcloning and three identical fragments were obtained from three independent pcr , lacking three continual bases ( ttc , encoding the pheamino acid ) compared with choe , thus it can be assumed that the new fragment , designated as choew , and choe belong to the same gene family , even it might be the natural mutation of choe
对片段进行克隆、亚克隆之后测序分析，发现与已克隆的来源于马红球菌的胆固醇氧化酶基因cboe有99的同源性；并且三次独立的pcr均得到相同的片段，所克隆的基因序列与choe相比，连续缺失三个碱基( ttc ) ，即相应的氨基酸序列缺失一个氨基酸( phe ) ，因此判断所克隆的基因片段与choe属同一基因家族或原有基因的天然突变体，命名为choew 。 |
