English translation for "胚成纤维细胞"
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- mdef
Related Translations:
胚茎: blastemafundamentumplumelet 胚后期: postembryonic periodpostembryonic stage
- Example Sentences:
| 1. | Cultivation of avian encephalomyelitis virus in cef 利用鸡胚成纤维细胞培养禽脑脊髓炎病毒的研究 | | 2. | Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast 66弱毒株在鸡胚成纤维细胞上的培养 | | 3. | Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection 亚群禽白血病病毒的鸡胚成纤维细胞系建立 | | 4. | In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv 本研究采用脂质体转染方法,将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞,经6轮蚀斑克隆、筛选、表达, pcr鉴定和dot - elisa检测,证明该重组病毒已构建成功,并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。 | | 5. | Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef ) 利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠,然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ,进行其分泌抗meq单克隆抗体( mcab )能力的筛选。 |
- Similar Words:
- "胚层细胞" English translation, "胚层形成" English translation, "胚层学说" English translation, "胚长生" English translation, "胚成长因子" English translation, "胚抽出物" English translation, "胚带" English translation, "胚胆碱" English translation, "胚的" English translation, "胚的代谢和水分含量" English translation
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