English translation for "莽草酸途径"
|
- shikimic acid pathway
Related Translations:
莽草酸: shikimic acidshikimicacid 莽草素: anisatinshikimeneshikiminsikimin 石莽草: capitate knotweed herb
- Example Sentences:
| 1. | Shikimic acid pathway 莽草酸途径 | | 2. | The epsp synthase is a necessary enzyme of the shikimate pathway and it is very conservative Epsp合成酶是莽草酸途径的一个关键酶,其成熟蛋白在进化中极为保守。 | | 3. | In microorganisms and plants the first step in the aromatic acid biosynthetic pathway is the condensation of pep and e4p , with the release of the phosphate of pep and the formation of dahp 在细菌和植物中,由公共途径莽草酸途径以及三个专一性途径分别合成苯丙氨酸、酪氨酸和色氨酸三种芳香族氨基酸。 | | 4. | When both genes were co - expressed in e . coli , the activity of ppsa varied from 2 . 1 - 9 . 1 fold comparing to control , but the activity of tkta was relatively stable ( 3 . 9 - 4 . 5 fold ) . whatever the two genes were expressed respectively or cooperatively , both could promote the production of dahp , the first intermediate of the common aromatic pathway , but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective . the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp , and what ' s more , when multigenes co - expressed , the recombinant which has coordinated enzymes activity is optimum 莽草酸途径的最优化和整体调控基因csra的敲除正是上述改变的分子基础,同时也为三种芳香族氨基酸的基因工程菌的构建打下了基础; 7 .在国内外首次实现了共同途径限制性底物关键酶ppsa刁无‘及arog与分支途径关键酶基因phea的串联高效表达,所构建的重组质粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分别比对照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比较协调一致; 8 .将ptga导入到筛选的基因敲除和基因替换菌株大肠杆菌31884 c甲b中,摇瓶发酵证实比以往所构建的基因工程菌株具有较高的phe产量和糖转化率率,分别为0 . 448 %和22 . 4 % 。 | | 5. | The product chorismate of this pathway is precursor to the synthesis of the aromatic amino acids phenylalanine , tyrosine , and tryptophan as well as other essential aromatic secondary metabolites such as ubiquinone , menaquinone and vitamin k . phosphoenolpyruvate and shikimate 3 - phophate are converted to 5 - enolpyruvlshimimate - 3 - phosphate and phosphate 芳香族氨基酸苯丙氨酸、酪氨酸、色氨酸和其它一些必需代谢物如泛醌、维生素k2类、维生素k的起始物是莽草酸途径所产生的分枝酸。 | | 6. | Quinic acid , used shikimate pathway in e . coli , it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell . double specific enzyme genes arog , qutb or three ones arog , qutb , arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions . modifications of e . coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch 利用大肠杆菌莽草酸途径合成新的代谢物奎尼酸,须在宿主细胞引入异源酶基因扩展代谢途径;串联表达酶基因,同时适量增加不同种属的多个关键酶酶量,改善限速反应;利用同源重组进行基因整合和基因破坏,改造染色体结构定向改变微生物代谢途径;目的是将碳代谢流最大程度的引向奎尼酸生成的方向。 | | 7. | We amplified ppsa and tkta from e . coli k - 12 by pcr and constructed recombinant plasmids of them in pbv220 vector containing prpl promoter . because of each gene carrying pl promoter , four productions of ligation were obtained , that are ppt - , ppt - , ptp - , and ptp - . the results of sds - page demonstrated that the bands at 84kd and 73kd were more intensive than the same ones of the controls 3 .从大肠杆菌中克隆了莽草酸途径关键酶基因aro刁、 aroc 、 arob及整体代谢调控基因csra及其侧翼序列;从质粒pet28a中克隆了kan抗性基因; 4 .构建了基因盒子aroaar口carobkan ,在其前面加入了强启动子ptac ,初步证明了pta 。 |
- Similar Words:
- "莽草实" English translation, "莽草实, 毒八角" English translation, "莽草素" English translation, "莽草酸" English translation, "莽草酸途经" English translation, "莽草油" English translation, "莽夫" English translation, "莽古" English translation, "莽古兴" English translation, "莽汉" English translation
|
|
|