| 1. | Purification and characteristics of an endo - beta - 1 , 4 - d - glucanase from aspergillus niger
葡聚糖酶的纯化和性质 |
| 2. | Determination of - glucanase activity in feed additives - spetrothoetric method
饲料添加剂-葡聚糖酶活力的测定分光光度法 |
| 3. | Purification and properties of an endocellulase from the thermophilic fungus chaetomium thermophile
葡聚糖酶的分离纯化及特性 |
| 4. | Study on screening a high - productive strain of dextranase and its basic conditions of flask fermentation
葡聚糖酶高产菌株的诱变筛选及其摇瓶发酵条件的初步研究 |
| 5. | The effect of adding neutral protease , papain and - glucanase on yeast autolysis were studied
摘要研究了外加中性蛋白酶、木瓜蛋白酶和-卜葡聚糖酶对酵母自溶的影响。 |
| 6. | Studies on transgenic oilseed rape brassica napus plants transformed with - 1 , 3 - glucanase and chitinase genes and its resistance to sclerotinia sclerotiorium
葡聚糖酶及几丁质酶基因的转基因可育油菜及其抗菌核病的研究 |
| 7. | It was thought to be a new gene encoding for chitinanse of b . subtilis . genome library methodology was adopted for cloning of endoglucanase encoding genes
虽然同源性较低，但酶活性表达较强，认为该基因是编码内切葡聚糖酶的一个新基因片段。 |
| 8. | The influence of cytosintetidemycin on activity of defense enzymes ( cat pod ppo pal , sod and 1 , 3 glucanase ) was studied , results suggested that the activity of defense enzymes was much more easily to be stimulated by cytosintetidemycin , and the level of 1 , 3 glucanase activity was also induced
摘要用嘧肽霉素处理辣椒植株后，测定了辣椒叶片组织内各种防御酶系（苯丙氨酸解氨酶、超氧化物歧化酶、过氧化氢酶、过氧化物酶、 - 1 , 3葡聚糖酶）的活性变化。 |
| 9. | Four colonies of transformed e . coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained . the gene fragment in these isolates was identified by the methods of plasmid processing . dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b . subtilis from 2599451 to 2812870 was 85 % , and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b . subtilis ) in genebank
测序并序列比较结果表明该基因片段同已发表的枯草芽孢杆菌几丁质酶和内切葡聚糖酶编码幕因的克隆及重组芽抱杆菌的构建glyb一apre之间的同源性是最高的，为35 % ;同bacz ’了了ussp . bp23ce1b 、 b . p朋刀us内切葡聚糖酶和b . pol理vxap一1 ， 4一内切葡聚糖酶的编码基因的同源性只有27 % 。 |
| 10. | The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii . two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium . the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome . at the same time ; compared agrabactenum - mediated method with gene gun method , the transformation frequency of the former was 16 . 7 % , while the latter was 50 % , so gene gun transformation method was suitable for long ya liiliwn
用携带有几丁质酶基因和- 1 、 3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经pcr和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16 . 7 ，基因枪法的转化率为50 ，因此可能基因枪转化法更适于龙牙百合的遗传转化。 |
