English translation for "蛋白h3"

histone h3

Example Sentences:

	1.	The activity of intact elongator complex is directed specifically toward the amino - terminal tails of histone h3 and h4 , the predominant acetylation sites are lysine - 14 of histone h3 and lysine - 8 of histone h4 完整的复合物可直接修饰组蛋白h3和h4尾部，其优先乙酰化位点是h3k14和h4k8 。
	2.	It has been shown that domain ia is responsible for cell recognition , domain ii is to be involved in translocation of the toxin across membranes , and domain hi catalyzes the adp - ribosylation of elongation factor2 , which arrests protein synthesis and results in cell death 人组蛋白h3是碱性核蛋白，富含精氨酸，在生理条件下， h3的精氨酸带正电荷，而dna的磷酸基团带负电荷，所以组蛋白和dna分子主要依靠静电引力相结合。
	3.	In this study , the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi , xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing , the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully . after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e . coli , the transformed hosts were induced by iptg , bysds - page and elisa analysis of host protein . the expression of the objective gene was detected , and it could account for 16 . 28 % of the total host protein . inclusion body was prepared from the incubating expression hosts induced by iptg 同时将原核表达载体pet - 28c用nco ， xho双酶切，回收酶切产物，将回收的酶切产物pea ， h3 ，载体进行连接，并转入dh5感受态细胞内，培养12 - 18小时后，挑取阳性菌落，经nco ， xho双酶切分析及pcr检测，筛选到阳性克隆，其质粒测序结果表明成功地构建了毒性基因缺失的pea与人组蛋白h3融合基因的原核表达载体。
	4.	Arginine rich human histone h3 is a kind of basic nucleosome protein . in the medium of physiological condition , by electrostatic interaction , histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge . in this paper , in order to establish a new technology of transfection , the functional domains la and ii gene segments of pea were lined with histone h3 gene segment , then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis 本实验在已成功获得的pea功能区a ，功能区基因片段与人组蛋白h3基因片段的克隆菌株的基础上，构建原核表达载体，将克隆质粒pmd - 18t - pea经nco ， mlu双酶切，回收酶切产物；将克隆质粒pmd - 18t - h3经mlu ， xho双酶切，回收酶切产物。
	5.	By treating the cells in s , g2 phase and prophase with histone deacetylase inhibitor tsa , and through the application of microscopic observation and western - blotting , we demonstrated that histone acetylation modification played important roles in the cell cycle regulation in physarum polycephalum , affecting the normal crossover of the checkpoints of s / g2 , g2 / m and mitosis exit 这些炎白的表达水平具有细胞周期依赖性，随着细胞周期的进行而发生变化。 tsa处理引起的多头绒泡菌s期、 gz期和前期细胞内组蛋白h3乙酚化水平的提高，改变了细胞内类cyclinbi蛋白、类。

Similar Words：

"蛋白c,补体蛋白" English translation, "蛋白c激活肽" English translation, "蛋白c缺乏症" English translation, "蛋白h2a" English translation, "蛋白h2b" English translation, "蛋白h4" English translation, "蛋白i" English translation, "蛋白n-乙酰葡糖胺基转移酶" English translation, "蛋白s测定" English translation, "蛋白s缺乏症" English translation