English translation for "选择培养"
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- selected culture
selective culture
Related Translations:
同期培养: synchronous culture 分离培养: fractional cultivationisolated cultureisolationseparation culture 培养人才: cultivate talentspersonnel training
- Example Sentences:
| 1. | Led to his adopting this younger brother
.于是他选择培养那个小伙子 | | 2. | After 4 times selected culture by ppt , 347 resistant calli were obtained . the ppt resistant calli were transferred to ms medium with 2 . 0 mg / l 6 - ba , 0 . 1 mg / l naa for regeneration
0mg l +蔗糖30g l的选择培养基中进行选择培养，每10天继代1次，连续选择4次，共得到347块抗性愈伤组织。 | | 3. | Trying hard to grasp and use this regularity , and accurately choose an efficient avenue for cultivating young skeleton teachers has a momentous practical meaning on the future of our country ' s development in the religious field
努力掌握和运用这一规律，并正确选择培养中青年骨干教职人员的有效路径，对于我国宗教界发展的未来具有重要的现实意义。 | | 4. | At present , the competition is very acute . so it is very important for long - term development of enterprises to enhance the human resources ( hr ) study , select and strain talent , use hr affectively
随着企业竞争的日趋激烈，目前，加强企业人力资源战略研究，为企业选择培养有用之才，将企业现有人力资源加以有效利用，对企业的长远发展具有十分重要的作用和意义。 | | 5. | To prepare the wild type mbl in prokaryotic system , a pair of primers was designed and synthesized , and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna . a recombinant prokaryotic expression vector , pet28 - mbl , was constructed by inserted the mbl gene into plasmid pet28 ( b ) , and after transfected it into ecoli bl21 ( de3 ) and induced with iptg , recombinant mbl protein was expressed successfully
本实验另选用了原核表达质粒pet28 ( b ) ，根据已构建好的含有mbl野生型基因的t载体pgem - mbl ，设计一对引物， pcr扩增mbl基因，凝胶回收，双酶切pcr产物和pet28 ( b )质粒， t4连接酶连接，转化大肠杆菌dhsa ，氨芋选择培养挑取克隆鉴定。 | | 6. | To construct eukaryotic expression vector of mbl gene with codon 54 point mutation , the target sequence in pgem - mbld plasmid , which conains mbl cdna with codon 54 mutant allele , was amplified by pcr . after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i , the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector , and the recombinant vector , named pci - mbl54 , was obtained . the pci - mbl54 , digested with restriction enzymes , was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本实验以ggc54gacmbl突变为研究对象，选用真核表达质粒pci - neo ，根据已构建好的含54位密码子突变型mbl基因t载体的结构，设计合成新的引物， pcr扩增54位密码突变型mbl基因，凝胶回收，双酶切pcr产物和pci - neo质粒， t4连接酶连接，将前者克隆至后者的sma和sal位点，转化大肠杆菌xl - 1blue ，氨苄选择培养。 | | 7. | Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg , and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti - p24 hybridoma cells are gained
方法：基因工程p24抗原免疫小鼠4次，历时2个月，取脾细胞与骨髓瘤细胞株sp2 0 ，用peg融合， hat选择培养和间接elisa筛选分泌抗p24抗体阳性的杂交瘤细胞，三次克隆化后得稳定分泌抗p24抗体的杂交瘤细胞株。 |
- Similar Words：
- "选择钮" English translation, "选择排除" English translation, "选择排流法" English translation, "选择排序" English translation, "选择排序法" English translation, "选择培养法" English translation, "选择培养基" English translation, "选择配分" English translation, "选择配份" English translation, "选择配合" English translation
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