| 1. | Abnormal development and expressing characteristics of quot; imprinting gene quot; in reconstituted embryos from porcine somatic nuclear transfer
猪体细胞核移植重构胚发育异常及印迹基因表达特征研究的进展 |
| 2. | There were great effects of the genetic constitution of es cell on the development of embryos reconstructed with es cells in intraspecies
在以胚胎干细胞为核供体，进行同种克隆时，胚胎干细胞的遗传内涵对重构胚的发育有很大影响。 |
| 3. | 6 - dmap + ccb + ethanol can effectively activate the reconstructed rat - mouse embryos . 5 ) 225 reconstructed embryos from 2 - cell to morula were transferred into 46 recipients
23 l6 dmap乙醇ccb能有效地激活种间核移植重构胚。 |
| 4. | Then the zona and polar body of oocytes treated with drug were removed by transferring into the m2 containing 0 . 5 % protease . the mouse fetal fibroblast cells were glued to the oocytes memb
该结果与重构胚的体外发育相符，表明正是这些己恢复微组装功能的化学去核卵母细胞才对其供体核具有重编程的支持能力。 |
| 5. | Meanwhile , there was different in the development of embryos reconstructed with various strain oocytes . the activation methods , passages and types of es cells were comparing in mouse cloning
在胚胎干细胞异种克隆中，比较了激活重构胚的不同方法、不同代数胚胎干细胞以及不同类型的供核细胞对异种重构胚的影响。 |
| 6. | 2 ) we got 55 . 08 % of fusion rate when reconstructed embryos were fused with 1500 - 1800v / cm voltage and 50ms impulse . in addition , a same result can be obtained with low voltage + length impulse or strong voltage + short impulse
41的卵母细胞达到成熟：在1500 1800v cm ， 30 40sin条件下，大鼠牛核移植重构胚能达到50厂0 55 |
| 7. | In order to know if the genetic constitution of recipient oocytes influenced the development of embryos reconstructed from es cell , oocytes of c57bl / 6j and kunming albino were used as recipient , respectively
为了了解受体卵的遗传内涵是否对胚胎干细胞重构胚发育也会产生影响，分别以c57bl 6j和昆明白小鼠的m期去核卵为受体，构建胚胎干细胞重构胚。 |
| 8. | In order to establish the technique of isolating es cell , and test the developmental ability of reconstructed embryos derived from es cell and oocytes of intra - or inter - species , mouse es cell were isolated from mouse blastocysts , and were used as donor nuclear , and were transferred into oocytes of various strains . the factors that influenced the development of reconstructed oocytes were determined . the developmental ability of embryos reconstructed with mouse es cells and rabbit oocytes was observed and analyzed
为了建立胚胎干细胞分离技术及了解其在同种和异种卵母细胞中的发育能力，首先从小鼠囊胚分离胚胎干细胞，并以它作为核供体，移植到同种不同品系去核的小鼠卵母细胞内，观察不同品系的卵母细胞对胚胎干细胞重构胚发育的影响。 |
| 9. | The ovaries were examined and the oviducts and uteri were flushed in vitro by laparotomy for maturated oocytes recovery . the results were as follows : in comparison with control group , fsh group and fsh + 30 % pvp group are more efficient ( p < 0 . 01 ) , whereas the two treatment groups in equal dosage have no significant difference ( p > 0 . 05 )
76 （ p 0刀5 ） ； nocodazole处理组与对照组卵裂率差异显著（ p 0刀5 ） ，而桑堪胚发育率无显著差异t o刀引；经川、叩、扣血清饥饿处理构建的重构胚，卵裂率和桑堪胚发育率无显著差异o 0刀引。 |
| 10. | Since the success of dolly , the first cloned sheep with the adult somatic cells as karyoplast donor , new approaches have been developed for nuclear transfer technology . here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture . enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
将培养10h的化学去核卵母细胞与供体成纤维细胞融合后lh 、 2h 、 3h ，分别有77 . 6 % 、 70 . 6 % 、 58 . 9 %重构胚的染色质发生凝集，其余胚胎的染色体则处于原核期;而只在融合后3h ， 27 . 9 %重构胚被标记出组装的纺锤体，且其中的同源染色体己经分离。 |
