English translation for "限制酶"

restriction endonuclease
restriction enzyme

Related Translations:

限制:  place [impose] restrictions on [to]; astrict; restrict; limit; confine; shut down on [upon]: 限制数量 limit to a number or amount; 年龄限制 age limit; 时间限制 time limit; 对 ... 实行限制 impose restrictio

Example Sentences:

	1.	After recovering fragment and cutting it by not i , we assay the sequence by electrophoresis 回收片段后用notl限制酶消化后电泳鉴定。
	2.	" for the discovery of restriction enzymes and their application to problems of molecular genetics 发现限制酶及其在分子遗传学方面的应用
	3.	The pcdnas and doc - 1r fragments have been reconstructed to form a new plasmid used by t4dna ligation enzyme on 25c 阳性菌落经扩增、提取质粒，再分别用notl 、 smal 、 bamhi限制酶进行酶切鉴定。
	4.	To make pipetting easier , we will use an enzyme master mix that already contains the appropriate buffers and the two restriction enzymes 为了能在吸取液体时操作方便，我们会利用已经含有适当缓冲溶液与两种限制酶的酵素反应混合液来操作。
	5.	In 1987 , the notion of a splicing system was introduced by tom head in [ 3 ] as a mathematical model of restriction enzyme digestion and subsequence religation in the recombination of dna molecules 1987年， tomhead发表了一篇论文，引入拼接系统( splicingsystem )的概念作为限制酶与dna (脱氧核糖核酸)作用、进行dna重组的数学模型。
	6.	Using a set of primers shared by endogenous and exogenous alvs ( a > b > c , d , e ) , gp85 gene was amplified by polymerase chain reaction ( pcr ) from dna extracted from common cef and exogenous alv positive samples 根据已经发表在ncbi的alv - a 、 b 、 e亚群的序列情况，分析多态性酶切位点，运用不同的限制酶bgl ， bamh ， ssp进行分型。
	7.	Based on the restriction endonuclease sites of the reference sequences of a , b , e subgroups published in ncbi , the dna was digested by restriction endonucleases bgiii , bamhi , sspi . the expected results were obtained when digested by bgiii specific to a subgroup or sspi specific to a and e subgroups 结果发现，扩增到的目的带只能用对a亚群特异的bgl限制酶或对a 、 e亚群特异的ssp限制酶切开，而不能用对b亚群特异的bamh限制酶切开。
	8.	Based on the structure and function analysis of hirudin , a potent thrombin inhibitor , and some platelet aggregation inhibitors , which contain the recognition sequence argglyasp as their functional motif , two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus . these chimera genes were constructed by pcr and inserted into the expression vector pet21a , the constructs were confirmed by restriction enzyme digestion and dna sequence analysis . these recombinant plasmids were transformed into 经限制酶消化和dna序列分析，证明两种重组质粒与设计完全一致。由于rgd -水蛭素嵌合基因上游连接了金黄色葡萄球菌蛋白a spa的信号肽序列，在iptg诱导下两种嵌合分子都获得了分泌表达，表达产物主要集中在细胞周质空间。
	9.	A eukaryotic expression vector pcdna3 . 1 - cptl was constructed by insert cp77 gene into the vector pcdna3 . 1 which is used in nucleic acid immunization . the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal . immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body 本研究通过限制酶将cpti基因片段从载体pbluel3上切下，插入真核表达载体pcdna3 . 1 ，构建了用于核酸免疫的真核表达载体pcdna3 . 1 - cpti ；质粒大量提取和纯化后，通过肌肉注射的方法免疫balb c小鼠，基因表达产物刺激小鼠机体产生免疫反应，从而获得了抗cpti蛋白的抗体。

Similar Words：

"限制贸易的联合" English translation, "限制贸易的实施" English translation, "限制贸易实施法" English translation, "限制贸易实施法庭" English translation, "限制贸易协定" English translation, "限制酶定位" English translation, "限制酶断片" English translation, "限制酶分析" English translation, "限制酶基因" English translation, "限制酶切dna" English translation