| 1. | Random amplified polymorphic dna rapd technique was applied to assess six 应用随机引物扩增多态性dna rapd方法,对我国水青网属 |
| 2. | Three natural population of l . sagitta , add up to 62 individual , with 17 random primers , 128 polymorphic loci 箭叶橐吾的三个自然居群,总计62个个体,使用17个随机引物,共扩增出128个多态位点。 |
| 3. | Five natural population of l . virgaurea , add up to 100 individual , with 17 random primers , 132 polymorphic loci 黄帚橐吾的五个自然居群,总计100个个体,使用17个随机引物,共扩增出132个多态位点。 |
| 4. | The procedure of ddrt - pcr applicable for silver staining was optimized by adjusting the amount of several critical reagents , including total rna , anchor primer , arbitrary primer , cdna and dntp 通过调整ddrt - pcr中总rna 、锚定引物、随机引物、 cdna和dntp等关键试剂的用量,优化了适用于银染检测的ddrt - pcr方法。 |
| 5. | Rapd technique was first used to analyze the phylogenetic relationships of 13 species or varieties in pueraria dc complex . seventy - two random primers were tested , among which 18 chosen primers generated clear polymorphic bands 从72个随机引物中筛选出了18个引物在13个不同的种和类型的葛资源中具有rapd多态性。 |
| 6. | The results showed that the higher genetic diversity and the genetic differentiation existed in the population of reaumuria soongorica , which was composed of 7 subpopulations and 136 individuals , generated by 15 primers . 71 loci had been detected and 69 were polymorphic 15条随机引物对红砂7个亚种群的136个个体进行扩增,共检测71个位点,其中多态位点69个。 |
| 7. | Twelve primers selected from 100 random primers amplified 254 frangments in 14 species of cymbidium , in which 243 segments are polymorphic . the rate of polymorphism was 95 . 7 % . and all the segments were ranging from 200bp to 2200bp 从100个随机引物中筛选出12个随机引物,对兰属植物14种材料进行随机扩增,共得到254条带,多态性带有243条,多态性百分率为95 . 7 。 |
| 8. | From a cross 18 x 28 ( each from the superior tree from xianju county and pan " an county in zhejiang province ) . 236 random oligonucleotide primers were screened and 33 primers were selected to generate rapd markers within a sample of 60 megagametophyte dnas 通过236个10bp的随机引物或组合对6粒黄山松种子胚乳组织dna进行pcr扩增,共筛选出33个多态引物用于作图。 |
| 9. | So it is very important to isolate and clone these genes in leymus multicaulis for utilizing in breeding . in this research , ddrt - pcr technique was used to study these genes and two common wheat - leymus multicaulis disomic addition lines were included 采用3条锚定引物和8条随机引物共计24种组合,获得了6480条耐病材料cdna扩增条带,其中91 . 8在不同处理条件下是非常一致的。 |