| 1. | In this paper , the karyotype and banding pattern of amphioxus b . belcheri tsingtauense were reported for the first time
本文首次报道了青岛文昌鱼染色体的核型和带型。 |
| 2. | The masp ( mannose - binding lectin - associated serine protease ) gene has been cloned by the method of degenerative pcr and the fragment of the pcr product is 630 bps in length
本文还利用pcr方法从青岛文昌鱼基因组dna中克隆masp (甘露聚糖结合凝集素相关丝氨酸蛋白酶)基因片段。 |
| 3. | 1 . chromosome number ( 2n ) and fundamental number ( fn ) the chromosome number and karyotype of amphioxus branchiostoma belcheri tsingtauense were studied using embryonic cells of the animal
染色体数目( 2n )和臂数( fn )用青岛文昌鱼的胚胎细胞作材料，对其染色体数目和核型进行研究。 |
| 4. | The reason for this is unknown at the present . possibly , cg - rich and at - rich regions in amphioxus dna are not as " rich " as those in the vertebrate dna , that is , amphioxus dna ha
虽然nogusa ( 1957 )认为b . belcheri存在xy型的性染色体，至于青岛文昌鱼的性染色体是xy型或zw型尚待深入研究。 |
| 5. | Rapd analysis was applied to study genetic diversity of amphioxus ( branchiostoma belcheri tsingtaoense ) in the test . total twenty - two samples , which are eleven males and eleven females were collected for use
本实验首次采用随机扩增多态dna ( randomamplifiedpolymorphicdna ， rapd )技术对青岛文昌鱼该群体雌雄各11条个体共22个样本进行遗传多样性检测。 |
| 6. | The counting of 150 well - spread samples revealed that the number of the chromosomes ranged from 18 to 38 , but the modal number of the chromosome was 36 , which was in proportion to 66 %
计数150个染色体分散好的中期相，发现染色体数目的变化范围是18至38条，长度为1 . 4至3 . 7 m ，染色体的众数是36 ，占150个中期相的66 ，说明青岛文昌鱼染色体的数目是36 。 |
| 7. | In this paper , the presence of lectins in the skin mucus of amphioxus was verified by the aggutination assay and the effects of physical and chemical treatments on hemagglutination activity of amphioxus skin mucus were tested by the means of heat treatment and ph treatment
本文利用青岛文昌鱼体表粘液凝集反应、热处理、 ph处理文昌鱼体表粘液及糖抑制文昌鱼体表粘液凝集反应等免疫学实验方法验证了青岛文昌鱼(且belcheritsingtaunese )体表粘液中的凝集素的存在并对其性质进行了研究。 |
| 8. | G - banding the gtg banding ( g - banding ) was carried out by the standard trypsin method with slight modification , which works well for protochordate because a good number of reproducible g - bands are consistently obtained from the embryonic cells of late blastulae and early gastrulae of amphioxus b . belcheri tsingtauense
G带型用稍作修改的标准的胰酶显带技术，进行gtg带纹的显示，它们能较好地显示头索动物青岛文昌鱼的晚期囊胚和早期原肠胚的中期染色体的g带，并且重复性好。 |
