| 1. | Cellular immune response after immunizing mice with eukaryotic plasmid carrying hcv hypervariable region 1related gene
丙肝病毒高变区1相关基因免疫小鼠诱导细胞免疫反应的研究 |
| 2. | The result of analysis for discrimination power from three hypervariable regions showed i higher than ii and ii higher than iii . the third hypervariable region was different among the ethnic groups we studied
分析各民族高变区i 、高变区n和高变区nl的鉴别能力，鉴别能力均为高变区i >高变区n >高变区hl ，而且各民族间多态性存在差异。 |
| 3. | There was a 100 % homology which was among sd - 3 / 98 , js30 / 99 and hk46 for the hv region of vp2 . it was demoastrated that the new wibdv strains gx8 / 99 had changed in both pathogenicity and vp2 gene
Sd l 97 、 sd 3 98 、 js 30 99相互之间及其与ibdv参考株hk46间均具有相当高的同源性，结果似乎说明ibdv毒株的致病性与vpz基因高变区变异的关系不大。 |
| 4. | Relatively , the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96 . 8 % - 97 . 2 % at dna or aa levels , than the homology among hk46 and 3 strains , strains gx8 / 99 more than 98 . 4 % - 98 . 6 % at dna or aa levels
为研究病毒的核酸分子结构与其致病性的关系，本研究选取了在致死率上不同的4个ibdv野毒株，比较了它们的vpz基因高变区共494个碱基序列。 |
| 5. | All the isolates in the study of genotype vii could be recoganized by the new cutting site at nt 872 by re hinf i which is absent in the genetic supgroups viia , viib , and group vi . the amino acid sequences of the coding region of the f gene were deduced
由核昔酸序列推导了f蛋白高变区1一125位氨基酸( aa ) ，通过分析比较发现了新的基因型和基因亚型与其他基因型毒株的差异还体现在aa位点的变化上。 |
| 6. | But the vvidbv strain gx8 / 99 that had been identified by gui only have saci side , and no sspl , the reason need farther research . according to the specific different sequence between cibdv and vvibdv in vvp2 gene . we also designed two sets of primers ( cibda + cibds and vvibda and vvibds )
另外，针对不同强弱毒株的1 v的pz高变区序列的差异：设计合成了vvibdv的型特异性引物（ ibda vvibds人建立型特异的rt pcr ，只需一次反应即可区分cibdv和vvibdv 。 |
| 7. | The most variable regions between hi and b strain reside in vp2 and vp3 overlap region ( 319 - 470 ) , which are supposedly exposed to the surface of the capsid . it is possible due to the selective pressure from host immune system . the largest divergence between the gpv - h1 and mdpv capsid polypeptides located between the start cordons of vp2 and vp3
H1株与gpv - b株氨基酸的差异集中在vp2 、 vp3重叠区的319 - 470位之间，这与预期暴露于病毒最表面的区域相吻合，可能是宿主免疫筛选导致的变异； gpv - h1株和mdpv之间还存在另一高变区，位于vp2和vp3起始密码子之间，这可能与两病毒有不同宿主域相关。 |
| 8. | A few years latter the research showed , vp2 hydrophilic region and antigen variant and virulence of ibdv had very affinity . in order to lucubrate difference of virulence and antigen , and validate correntness of sequencing result , the test adopted sandwich enzyme - liked immunosorbent assay ( elisa ) and antigen caprure - elisa to determine virulence and antigen . and validate correntness of above - mentioned result
而近几年的研究表明， vp2高变区与ibdv的抗原变异和毒力强弱有密切的关系，为进一步了解强弱毒株毒力和抗原的差别，并进一步证实测序结果的正确性，本试验采用双抗夹心elisa和ac - elisa的方法分别对其毒力和抗原进行了测定，并通过动物试验证实了，其结果与序列分析结果基本一致。 |
| 9. | 24 nucleotides differences existed between the vp3 gene of hland b isolates are mainly distributed from 312bp to 4l7bp and 79lbp to i276bp , the two regions might be mainly the high mutation regions . the deduced amino acid sequence of hl isolate has nine amino acids differences with gpv b jsolate which locates mainly in the region from 266aa to 417aa
除个别突变点外，大多数变异集中在两个区域， vp3基因5 ’端312bp - 417bp区和3 ’端791bp - 1276bp区，由该序列推导的氨基酸序列与gpvb株有9个氨基酸发生改变，集中在266位和417位氨基酸之间，这一区域可能是vp3基因的高变区。 |
| 10. | The 301 - 330bp hypervariable ii - control - region sequence in mitochondrial ( mt ) dna genome from 18 individuals of one subspecies of great bustard ( o . t . dybowskii ) from breeding areas of northwest of songliao plain , southwest of hulunbeier plateau and wintering area of shandong yellow river nature reserve in china were sequenced and population analysis was conducted to assess the level of genetic diversity
本文采集了中国境内松辽平原西北部繁殖地、呼伦贝尔高原西南部繁殖地及山东黄河三角洲自然保护区等地区大鸨东方亚种( o . tdybowskii ) 18个个体，对线粒体dna控制区高变区的部分序列进行了测定和群体分析，以评价当前中国境内的东方亚种的遗传多样性水平。 |
