Optimization of a fermentation medium for linoleic acid isomerase production with lactobacillus acidophilus 嗜酸乳杆菌发酵产亚油酸异构酶培养基条件的研究
3.
The results showed that citrate sodium added to ferment liquid could decrease the stressing effect and enhance density of lactobacillus acidophilus during fermentation 结果表明，在发酵过程中，添加一定量的柠檬酸钠可乳酸对嗜酸乳杆菌的胁迫作用，从而提高了活菌密度。
4.
In order to increase the number of living bacterium , the proper alkali or alkali salt was added to ferment liquid to eliminate stressing effect of organic acid on lactobacillus acidophilus 摘要采用向发酵液中添加适当的碱或碱性盐等物质的方法，消除了影响嗜酸乳杆菌生长的有机酸的用，达到单位体积的发酵液内含有更多活菌体的目的。
5.
Ten potential probiotics stains were tested in this experiment . including two lactobacillus acidophilus strains fbi and a878 , two bifidobacteria strains pba and inf , four enterococcus strains ml , pb2 , a30 , a31 . one bacillus abticism2 , one clostridium butyricum strain was isolated from commercial probiotics by using tpy 本试验共测定9株潜在益生菌，其中pb1和a878为嗜酸乳杆菌； inf和pba为双歧杆菌； m1 、 pb2 、 a30 、 a31为链球菌； m2为枯草芽孢杆菌。
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The results suggested that l . acidophilus pblgrew well with enterococcus strains ml , pb2 , a30 , a31 . and had effective inhibition on the pathogens , the best combination were l . acidophilus pb1 with enterococcus m1 , a30 . l . acidophilus a878 grew not well with enterococcus strains ml , pb2 , a30 , a31 . the growth inhibition of enterpathogens was lower than l . acidophilus pb1 在pb1与各球菌混合培养的基础上，再和inf和pba混合培养，结果发酵液中inf和pba的生长受到限制，结果表明，在体外pba和inf很难与需氧菌共生。
7.
But four enterococcus grew quickly and hadn " t delaying period , the final period of logarithm growing of all tested strains were 12 hour for i acidophilus pb1 and a878 ; 24 hour for bifidobacteria pba and inf ; 8 - 10 hour for enterococcus ml , pb2 , a30 , a31 and bacillus abticism2 the growth inhibition of escherichia coli , shigellosis , salmonell by the fermented liquor of eight probiotics bacteria was tested 确定各菌株对数生长期末期分别为： pb1和a878 ： 12h ； inf和pba ： 24h ； pb2 、 m1 、 a30 、 a31 、 m2 ： 8 - 10h 。采用牛津杯法测定八种潜在益生菌对大肠杆菌、痢疾杆菌、沙门氏菌的抑制性，结果表明，嗜酸乳杆菌pb1 、 a878和双歧杆菌pba 、 inf具有较强的抑菌能力，对三种致病菌能够形成明显的抑菌圈，而且对三种致病菌的抑菌能力明显不同。
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The inhibition mechanisms of pb1 , a878 , pba , inf were some organic acids and antibacterial substance in fermented liquor . l . acidophilus strains pb1 , a878 mixed with enterococcus strains ml , pb2 , a30 , a31 respectively in the ratio of 1 : 1 in the mrs cultures , co - cultural and single liquor inhibition to the enterpathogens were tested in the terminal fermentation 将pb1和a878分别与m1 、 pb2 、 a30 、 a31以1 : 1的比例混合发酵后，测定联合菌株对致病菌的抑制性，结果表明pb1与各球菌联合后，可以互利生长，其中复合较好的为pb1与m1和a30 。
9.
L . acidophilus strains pb1 , a878 mixed with enterococcus strains m1 , pb2 , a30 , a31 . the amounts of cholesterol removed from growth medium were tesed after anaerobical incubation at 37 cthe amount of cholesterol assimilated higher 40 % by pb1 + m1 , pb1 + pb2 a878 + a30 , a878 + a31 . the result indicated that pblmixed with ml , pb2 and a878 mixed with a30 , a31 can assimilate cholesterol effectively 将pb1 、 a878分别和各球菌m1 、 pb2 、 a30 、 a31接种于高胆固醇培养基中，在有胆汁和厌氧条件下， 37混合培养16 - 18h ，测定复合菌株和单菌株降低胆固醇的效果，以未接菌培养基为对照，结果表明， pb1与m1及pb2 ； a878与a30及a31的协同作用较好，降培养基中胆固醇的量都高于40以上。
10.
On the base of i . acidophilus strains pb1 mixed with enterococcus strains m1 , pb2 , a30 , a31 , bifidobacteria pba and inf were added in the culture . the results suggested pba and inf were limited on the co - cultures , its indicated that pba and inf grew badly with other probiotics in vitro each mice were given 0 . 5ml fermentative liquor of probiotics strains pbl + a30 , pba , pb1 , m1 , pb1 + m1 , pb1 + a30 for one month 将几株体外性状好的菌株pba 、 pb1 、 m1 、 pb1 + m1 、 pb1 + a30 ，每天以0 . 5ml每只发酵液的量灌胃给正常小白鼠，时间30天，测定小鼠免疫器官指数和血清中细胞因子的变化，并观察小鼠脾脏的变化。
