Studies on the biological charcteristics of alcaligenes xylosoxidans 木糖氧化产碱杆菌生物学特性研究
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Association nitrogen fixation of rice inoculated with ammonia resistant engineering strain alcaligenes faecalis 粪产碱菌耐铵工程菌与水稻联合共生固氮作用
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In this study a microbial system for biphenyl biodegradation is set up in order to investigate the effects of surfactants on biodegradation of hydrophobic organic compounds . four strains which can grow on biphenyl as the sole carbon and energy sources are selected out , in which alcaligenes eutrophus dj1 and pseudomonas sms02 are chosen as degradation strains . after studing the bioavailability of three nonionic surfactants ( op - 10 , tween - 80 , and triton x - 100 ) , they are added into the biodegradation system of biphenyl 为了考察表面活性剂对疏水性有机污染物生物降解的影响，本论文选择联苯作为模拟研究体系，筛选、驯化得到四株可利用联苯作为碳源生长的菌株；考察了联苯降解菌株对三种非离子表面活性剂op - 10 、 tween - 80 、 tritonx - 100的生物可利用性；用高效液相色谱法测定了非离子表面活性剂对联苯的增溶曲线；较系统地研究了加入非离子表面活性剂对联苯生物降解速率的影响。
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Considering alcaligenes faecalis pencillin g acylase ( afpga ) , which possesses the attractive characteristics for beta - lactam antibiotics conversions , the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga . both the constructed plasmids psmlfpga and pkkfpga contained the pga gene , trc promoter and rrnb transcript terminator , differ in the replicon and antibotic marker , pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker . while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker 本文将粪产碱杆菌青霉素g酰化酶( afpga )基因构建表达载体pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc启动子、青霉素g酰化酶基因、 rrnb转录终止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷贝复制子；而psmlfpga含有四环素抗性基因和p15a中拷贝复制子。
