| 1. | Angiogenesis promoting effects of chinese herbal medicine for activating blood circulation to remove stasis on chick embryo chorio - allantoic membrane
活血化瘀中药对鸡胚绒毛尿囊膜血管生成的影响 |
| 2. | The a / pr / 8 / 34 ( hln1 ) adapted to mouse lung was propagated in allantoic cavity of 9 - day - old embryonated chicken eggs and titered by hemagglutinin test , and the tilers was 1 : 320
用9日龄鸡胚培养人流感病毒的鼠肺适应株户jp侧8 / 34 ( hini ) ，通过血凝实验测得病毒滴度为1 : 320 。 |
| 3. | Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
用qiagen的rneasyrna试剂盒提取病毒rna后，逆助兴才李医‘笋脂硕圣‘老‘才转录合成cdna 。 |
| 4. | Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
用qiagen的rneasyrna提取试剂盒按说明提取，提取的rna逆转录合成cdna ， cdna为pgr反应扩增的模板，特异性的引物是针对各个基因片段设计的。 |
| 5. | Two ndv strains , lasota and f48e9 , were propagated in 9 - day - old embryonated specific - pathogen - free ( spf ) eggs respectively for 36 hours at 37 , then these chicken embryos were placed at 4 for 12h before the allantoic fluid was gained
首先，将ndvlasota株和f48e9株分别接种于9日龄spf鸡胚，于37孵化36h ，置4 12h后收获鸡胚尿囊液。 |
| 6. | The chickens and chicken embryos were inoculated with variant serotype isolate e of infectious bursal disease virus using cloacal , nosal routes or via the allantoic cavity route , and the histopathological features of the bursa of fabricius of the ibdv _ infected chickens at various intervals of time were systematically investigated
本试验全面而系统地观察了传染性法氏囊病病毒变异e株，通过泄殖腔、鼻腔和尿囊腔接种雏鸡和鸡胚后不同时间法氏囊的组织形态学变化。 |
| 7. | Heamagglutination tests were applied to detect virus in allantoic fluid of chicken embryos which were infected by b95 gathered from the vaccinated chickens " cloacal and oral cavity . the results show that the virus may be detected from 2 days to 11 days after the chicken being vaccinated . the hi antibodies were measured by heamagglutination inhibition tests . there is no significant difference between the immunized and the control chickens which were fed in one case . chickens were immunized with b95 by different immunization meathods or with different vaccines by the same meathod . lt is demonstrated that eyedrop , drinking water , spray or muscle injection all can stimulate good effects , but eyedrop and spray seem to be the best meathods . b95 immunized chicken have relatively higher hi titers and it also can last for a longer time than others
但如果两者相隔10天以上免疫， b95免疫不受h120的影响；如果同时免疫b95和h120 ，加大b95的免疫剂量也能获得良好的免疫效果。用棉拭子采b95免疫鸡口腔、泄殖腔的分泌液，检测其中病毒的存在，结果免疫后2 11天鸡口腔和泄殖腔中均有病毒的存在，说明b95免疫鸡带毒时间长。研究结果表明， b95具有不受母源抗体干扰、 hi抗体产生快、水平高、持续时间长、同居扩散性强等特点，因此b95是一株优良的、具开发前景的新的新城疫疫苗株。 |
| 8. | Newcastle disease virus ( ndv ) strain 695 , a thermostable nature avirulent strain , were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene , a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用从国外引进的新城疫热稳定性天然弱毒b _ ( 95 )株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna ，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应( rt - pcr )扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t - a克隆技术将其克隆到pgem - t - easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。 |
| 9. | Abstract : in ureide - transporting legumes ， ureides ( allantoin and allantoic acid ) are major transportform of biological fixed nitrogen . the distribution ， properties ， structure and regulation of plant allantoinase were reviewed in detail . the advances in the recent studies on allantoate amidohydrolase and ureidoglycolate amidohydrolase were introduced . and the works desired to be done were pointed out
文摘:全面综述植物酰脲降解代谢酶的研究现况，概述植物尿囊素酶的分布、性质、结构，以及调节控制，并简介植物尿囊酸酰胺水解酶和脲基乙醇酸酰胺水解酶的研究近况，同时指出待研究的方向。 |
| 10. | The amniotic fluid and allantoic fluid of chicken embryo have been analyzed and the resonances of most substances in them were assigned by 1 - dimention and 2 - dimention nuclear magnetic resonance ( nmr ) methods . this work could be the basis of quantitative analysis of metabolites and studying the metabolites changing between the amniotic and allantoic fluid during the hatching process . also , it provided a new way to study the process of embryo developing
用一维及二维液体高分辨核磁共振( nuclearmagneticresonance ， nmr )方法综合分析了鸡胚羊水和尿囊液的成分，对其中的大多数谱峰进行了归属，发现了一些未经报道的小分子代谢物，为研究胚胎发育过程中各个胚囊中体液的交换和小分子的代谢以及进一步对发育过程中代谢物含量变化的定量研究打下了基础，并为研究胚胎发育的过程提供了新的思路。 |
