( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed ( 2 )在翻译水平上通过pcr扩增的方式在t - pa基因起始密码子处添加了植物翻译起始共有序列aaca ，构建了植物表达载体pbet 。
4.
The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest . then the recombinant plasmid was identified by endonuclease analysis , pcr ampliation and dna sequencing . the report showed that the recombinant plasmid had right open reading frame 重组质粒经酶切鉴定， pcr鉴定和测序，结果证实猪肺炎支原体黏附因子p97基因的抗原决定簇r1区定向插入了质粒pet - 32a ( + ) ，且阅读框架正确。
5.
After the amplication of primer s103 , there was a specific fragment about 850bp in t2201 ; after the amplication of primer s74 , there were two new fragment about isoobp and 400bp in variant t2202 . the amplified prauct of si 18 had a 480bp new fragment in variant t2204 . in variant t2203 there were two specific fragments about 980bp and 550bp after ampliation of primer s64 经引物s103扩增，在变异体t2201中出现850bp的特异片段；引物s74在变异体t2202中扩增出1500bp和400bp的两条新带；引物s64在变异体t2203中扩增出两条新带，大小分别为980bp和550bp ；引物s118在变异体t2204扩增出一条480bp的新带。
