| 1. | The results indicted that ecbp21 is a kind of secretive protein and ecbp21 is an apoplast polypeptide
由此进一步证实ecbp21确为一种质外体多肤。 |
| 2. | Apoplast is an important compartment outside of plasma membrane of the plant cell and is the source of various primary signals
摘要细胞质膜以外的质外体是植物细胞的重要组成部分，质外体是植物细胞的重要信号源和细胞器。 |
| 3. | Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco . the results of fluorescent detection showed gfp localization in the apoplast
Elisa结果显示，马铃薯蛋白酶抑制剂基因的信号肽序列使crylac基因在转基因烟草中的表达量显著提高。 |
| 4. | To guide the cam - binding peptide into apoplast space , the signal peptide from brassica campetris pollen coat protein slr1 ? ps was employed . it has total 26 amino acid , and is a very classic form of signal peptide
为了把cam结合小肽带到质外体，本实验选择了野生芸苔属植物( brassicacampetris )花粉胞壁蛋白slr1 - bps的信号肽。 |
| 5. | This work laid a foundation for elucidating the functions of ecbp21 by means of molecular biology . further studies about ecbp21 may be benefit to elucidating the mechanism of apoplast calmodulin or enriching
本实验结果也为利用分子生物学方法研究ecbp21的生物学功能奠定了基础，并将有助于阐明胞外cam的作用机制及丰富植物质外体多肤的研究。 |
| 6. | Calmodulin , as a versatile ca ~ ( 2 + ) sensor , plays many important biological functions . meanwhile , our previous experiments suggested that calmodulin exists in the apoplast of plant cells and has many physiological functions
多年来，我室一系列研究表明植物细胞外普遍存在cam ，并且细胞外cam作为质外体多肽，发挥许多重要的生物学功能。 |
| 7. | In our laboratory , a series of experiments has provided evidence that the presence of cam in the apoplast area is universal , and extracellular calmodulin plays an important role in the plant growth and development as a polypeptide signal
我室多年的研究证实植物细胞外也普遍存在cam ，并且可能作为一种多功能肽类信使在植物生长发育过程中发挥重要作用。 |
| 8. | The result also demonstrates that althought the apoplast ca2 + concentration is 10 - 10 times higher than that of the intracellular ca2 + , the activation of extracellular cam is regulated by ph duing signal transdction , vhich furthermore provides an important evident for the apoplast cam signal transdction mechanism
这可能与不同物种及亚型的钙调素本h的细v特点有关。更为重要的是，随着ph的变化，两种ca 1与ca ‘的结合常数均随ph降低而眸任。 |
| 9. | Our previous study suggested that the calmodulin ( cam ) existed in apoplast of plant cells and had various biological functions in plants . based on these studies , we put forward a view that the biological functions of extracellular c am m ay act a s a p olypeptide s ignal
我室多年的研究工作不仅证实植物细胞外cam的普遍存在，而且表明细胞cam具有多种生物学功能，并提出了细胞外cam可能作为多功能肽类信使在植物生长发育中发挥生物学功能的观点。 |
| 10. | In order to understand the apoplast cam gene and the relationship between the apoplast cam and cam isoforms , we intended to get transgenic plants harboring soybean calmodulin isoform genes ( scams ) fused to gfp as a reporter and study the subcellular localization of scams
为了进一步利用分子生物学方法在基因水平上研究胞外cam的存在，同时研究胞外cam与cam亚型之间的关系，本论文利用绿色荧光蛋白( gfp )作为报告基因研究了大豆cam基因家族( scams )的亚细胞定位。 |
