| 1. | Diagnosis and genetic analysis of resistance to cauliflower mosaic virus in brassica crops which was transformed with camv gene vi
芸薹属蔬菜植株抗病性鉴定及其遗传分析 |
| 2. | And then the ced - 9 gene was inserted into plant expression vector pbi121 under the control of camv 35s promoter
在此基础上再构建重组表达载体pbi - ced9 ，将ced - 9基因置于camv35s启动子控制之下。 |
| 3. | We confirmed that the cloned gene dreb1c was really transformed into arabidopsis by pcr method , in which sequences of camv 35s were used as forward primer
提取转基因植株的基因组dna ，进行pcr反应，证明了dreb1c确实转入拟南芥中。 |
| 4. | 2 . in order to express chs in plant , we constructed plant expression vector p1301b q c . first added camv 35s promoter and nos terminator to chs by one median clone
( 2 )为了能在在植物中表达( chs ，通过一步的中间克隆，将chs连上camv35s启动子和nos终止子。 |
| 5. | Up to date , the cauliflower mosaic virus ( camv ) 35s promoter and its derivatives are used commonly . no report show transgenic plants expressed badh from halophyte and using its own promoter or other strong promoters
目前在遗传工程中所用的启动子多为35s ，还没有高效胁迫诱导启动子的实际运用的报道。 |
| 6. | This gene , artificially put under the control of camv 35s , was introduced into tobacco with the aid of agrobacterium , and the transgenic plants obtained were identified by gus staining , pcr and southern blot analysis
将arge与组成型启动子camv35s相连，构建植物表达载体，通过农杆菌介导转化烟草。经过gus染色、 pcr及southern鉴定获得了转基因植株。 |
| 7. | By blue - white screening and sequence analysis , we obtained the positive clone . 2 . we constructed plant binary expression vector carrying fusions of the enhanced camv 35s promoter and dreb1c ( 35s : dreb1c ) in the sense orientation
我们从拟南芥基因组中克隆了dreb1c基因全长，并将其连接到中间载体pgem - t - easy上，进行了测序验证，结果显示所克隆的dreb1c序列完全正确。 |
| 8. | To target this mitochondrial enzyme into chloroplast , the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene , whereas expression of the chimeric gene was driven by the camv 35s promoter
Pchlsod质粒含有烟草mnsod基因的cdna序列，与豌豆核糖体小亚基叶绿体引物肽( tp )的编码基因序列构成融合基因，由35s启动子调控。 npt基因为选择标记基因， pgv2260为辅助质粒。 |
| 9. | Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121 , which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter , was used in the establishment of the genetic tranformation of white clover
选用苗龄4 5天的带柄子叶作为外植体，先将外植体预培2天，再与根癌农杆菌a311共培养3 4天后，转入附加有40mg l ~ ( - 1 )卡那霉素和400mg |
| 10. | Driven by the cauliflower mosaic virus ( camv ) 35s promoter , the er - shsp over - expressed constitutively . the growth and the phenotype of transgenic plants can be used for researching the function of er - shsp in improving tomato ' s cold resistance and the er - shsp chaperones function in vivo . after degested by kpnl and xbal enzymes from the pbs - er plasmid , the gene er - shsp - lehsp21 . 3 was inserted into the prokii vector to construct an eukaryotic expressing vector
利用基因工程方法，将内质网小分子热激蛋白基因( endoplasmicreticulum - locatedsmallheatshockproteingene , er - shspgene ) - lehsp21 . 3导入到番茄体内，使之在植物体中组成性表达( constitutiveexpression )内质网小分子热激蛋白( er - shsp ) ，观察转基因番茄在低温条件下的生长和表型反映，研究er - shsp在提高植物耐寒性中的作用，同时为体内研究er - shsp的分子伴侣机制提供依据。 |
