The present study , ( d by using immunohistochemical single , double or triple staining method , showed the expression , relationship and distribution pattern of fos - protein , gfap or th in rat cns , investigated the plastic response and relationship of rat lumbar spinal cord as and neurons to pain induced by the unilateral tibia and fibula fracture ; ( 2 ) by using a double immuno - electron - microscopic method , investigated the ultrastructural characters of junction areas between neurons and as in the dorsal horn of rat lumbar spinal cord following the unilateral tibia and fibula fracture ; ? after intrathecal application of the carbenoxolone , a gap junction blocker , recorded the paw withdrawal thermal latency and compared with control 在脑干gfap - li星形胶质细胞主要分布于mvz内的孤束核( nts ) 、腹外侧延髓( vlm )以第四军医大学博士学位论文及两者之间的中间带（ irt ）上。三叉神经脊束核尾侧亚核（ vc ） 、外侧楔柬核（ ecu ） 、蓝斑（ lc ） 、臂旁外侧核（ lp ） 、中缝大核（ rmg ） 、中脑导水管周围灰质腹外侧区（ vipag ） 、中缝背核（ dr ）等部位也出现一定数量的gfap阳性细胞。 f 。
